IL12P40 recombinant protein

IL12P40, 23-328aa, Human, His tag, Baculovirus

Cat. Num. AAA204259

• Gene Names: IL12B; CLMF; NKSF; CLMF2; IMD28; IMD29; NKSF2; IL-12B
• Applications: SDS-Page
• Purity: > 90% by SDS-PAGE
• Synonyms: IL12P40; 23-328aa; Human; His tag; Baculovirus; interleukin 12 subuit beta; CLMF; NKSF; CLMF2; NKSF2; IL-12B; IL12P40 recombinant protein

• Host: Baculovirus
• Purity/Purification: > 90% by SDS-PAGE
• Form/Format: Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 2mM DTT, 20% glycerol, 100mM NaCl, 0.1mM PMSF
• Concentration: 0.25mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: ADPIWELKKD VYVVELDWYP DAPGEMVVLT CDTPEEDGIT WTLDQSSEVL GSGKTLTIQV KEFGDAGQYT CHKGGEVLSH SLLLLHKKED GIWSTDILKD QKEPKNKTFL RCEAKNYSGR FTCWWLTTIS TDLTFSVKSS RGSSDPQGVT CGAATLSAER VRGDNKEYEY SVECQEDSAC PAAEESLPIE VMVDAVHKLK YENYTSSFFI RDIIKPDPPK NLQLKPLKNS RQVEVSWEYP DTWSTPHSYF SLTFCVQVQG KSKREKKDRV FTDKTSATVI CRKNASISVR AQDRYYSSSW SEWASVPCSH HHHHH
• Sequence Length: 328

• Applicable Applications for IL12P40 recombinant protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Endotoxin Level: < 1.0 EU per 1 microgram of protein (determined by LAL method)
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for IL12P40 recombinant protein

IL12P40 (interleukin 12 subunit beta), also known as natural killer cell stimulatory factor 2, or cytotoxic lymphocyte maturation factor 2, p40, belongs to the type I cytokine receptor family. This protein is a cytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine-activated killer cells, and stimulate the production of IFN-gamma by resting PBMC. Interleukin 12 is a disulfide-linked heterodimer composed of the 40 kD cytokine receptor like subunit encoded by this gene, and a 35 kD subunit encoded by IL12A. This cytokine is expressed by activated macrophages that serve as an essential inducer of Th1 cells development. Recombinant human IL12P40 protein, fused to His-tag at C-terminus, was expressed in Hi-5 cell using baculovirus expression system and purified by using conventional chromatography.

Product Categories/Family for IL12P40 recombinant protein

Hormones & Cytokines

References

Oppmann B., et al (2000) Immunity 13:715-725 (2000); Presky et al. (1996) Proc. Natl. Acad. Sci. u.S.A. 93 (24): 14002-7.

NCBI and Uniprot Product Information

• NCBI GI #: 24497438
• NCBI GeneID: 3593
• NCBI Accession #: NP_002178
• NCBI GenBank Nucleotide #: NM_002187.2
• UniProt Accession #: P29460
• Molecular Weight: 35.8kDa (315aa)
• NCBI Official Full Name: interleukin-12 subunit beta
• NCBI Official Synonym Full Names: interleukin 12B
• NCBI Official Symbol: IL12B
• NCBI Official Synonym Symbols: CLMF; NKSF; CLMF2; IMD28; IMD29; NKSF2; IL-12B
• NCBI Protein Information: interleukin-12 subunit beta
• UniProt Protein Name: Interleukin-12 subunit beta
• UniProt Gene Name: IL12B
• UniProt Synonym Gene Names: NKSF2; IL-12B; CLMF p40; NKSF2

NCBI Description

This gene encodes a subunit of interleukin 12, a cytokine that acts on T and natural killer cells, and has a broad array of biological activities. Interleukin 12 is a disulfide-linked heterodimer composed of the 40 kD cytokine receptor like subunit encoded by this gene, and a 35 kD subunit encoded by IL12A. This cytokine is expressed by activated macrophages that serve as an essential inducer of Th1 cells development. This cytokine has been found to be important for sustaining a sufficient number of memory/effector Th1 cells to mediate long-term protection to an intracellular pathogen. Overexpression of this gene was observed in the central nervous system of patients with multiple sclerosis (MS), suggesting a role of this cytokine in the pathogenesis of the disease. The promoter polymorphism of this gene has been reported to be associated with the severity of atopic and non-atopic asthma in children. [provided by RefSeq, Jul 2008]

Uniprot Description

Cytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine-activated killer cells, and stimulate the production of IFN-gamma by resting PBMC.