Typical Testing Data/Standard Curve (for reference only)
Interleukin-6 Soluble Receptor ELISA Kit | IL-6 sR elisa kit
Interleukin-6 Soluble Receptor (IL-6 sR)
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: This IL-17A enzyme linked immunosorbent assay (ELISA) applies a technique called a quantitative sandwich immunoassay. The microtiter plate provided in this kit has been pre-coated with a monoclonal antibody specific to IL-17A. Standards or samples are then added to the appropriate microtiter plate wells. A biotin-conjugated antibody preparation specific for IL-17A was added and incubated. IL-17A, if present, will bind and become immobilized by the antibody pre-coated on the wells. The microtiter plate wells are thoroughly washed to remove unbound IL-17A and other components of the sample. Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Avidin is a tetramer containing four identical subunits that each has a high affinity-binding site for biotin. The wells are thoroughly washed to remove all unbound HRP-conjugated Avidin, and a TMB (3,3'5, 5' tetramethyl-benzidine) substrate solution is added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain IL-17A, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in colour. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the colour change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. In order to measure the concentration of IL-17A in the samples this kit includes two calibration diluents (Calibrator Diluent I for serum/plasma testing and Calibrator Diluent II for cell culture supernatant testing.) According to the testing system, the provided standard is diluted (2-fold) with the appropriate Calibrator Diluent and assayed at the same time as the samples. This allows the operator to produce a standard curve of Optical Density (O.D) versus IL-17A concentration (pg/mL). The concentration of IL-17A in the samples is then determined by comparing the O.D. of the samples to the standard curve!!Background/Introduction: Interleukin-17A (IL-17A) is a secreted, homodimeric glycoprotein linked by disulfide link with a molecular mass of 35 Kilo Dalton. The cytokine is the proto-type of a newly discovered pro-inflammatory cytokine family which consists of IL-17A, IL-17B, IL-17C, IL-17D, IL-17E (IL-25) and IL-17F. All the IL-17s have similar structure with four highly conserved cysteine residues and same 3 dimensional structures, which distinguish this family from other cytokines. IL-17 is produced by a group of CD4 T help cells termed Th17 cells. In addition to previous discovered Th1 and Th2 cells, the Th17 cells consist in the third group of T help cells. IL-23, a growth and stabilization factor was the first cytokine that was found to involve with the development of Th 17 cells. Later on, other cytokines including TGF-beta, IL-6, IL-21 were also found to contribute to the procedure. The transcription factors implicated in the procedure were identified as STAT3, RORgt, and RORa. IL-17, like interferon-gamma, is a potent mediator of delayed-type reaction. IL-17 induces the secretion of many other pro-inflammatory cytokines and chemokines such as IL-6, IL-8, GM-CSF, GCSF, TGF-beta, TNF-alpha, GRO-alpha, MCP-1, and stimulates the expression of NFkappaB, mitogen activated protein kinase, and prostaglandings from many cell types. By stimulating cytokine production and recruiting monocytes neutrophils to site of inflammation, the cytokine exerts its effect in response to tissue damage and invasion. Excessive expression of IL-17 was found to associate with autoimmune diseases and chronic inflammatory diseases such as multiple sclerosis, rheumatoid arthritis, psoriasis, inflammatory bowel diseases, tissue allograft rejection, allergic asthma and infectious diseases at the mucosal tissue. This IL-17A ELISA is a 3.5-hour solid phase immunoassay readily applicable to measure IL-17A levels in serum, plasma, cell culture supernatant, and other biological fluids in the range of 0 to 1000 pg/mL. It showed no cross reactivity with other cytokines tested. This IL-17A ELISA is expected to be effectively used for further investigations into the relationship between IL-17A and the various conditions mentioned.
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a signal transducer shared by many cytokines, including interleukin 6 (IL6), ciliary neurotrophic factor (CNTF), leukemia inhibitory factor (LIF), and oncostatin M (OSM). This protein functions as a part of the cytokine receptor complex. The activation of this protein is dependent upon the binding of cytokines to their receptors. vIL6, a protein related to IL6 and encoded by the Kaposi sarcoma-associated herpesvirus, can bypass the interleukin 6 receptor (IL6R) and directly activate this protein. Knockout studies in mice suggest that this gene plays a critical role in regulating myocyte apoptosis. Alternatively spliced transcript variants have been described. A related pseudogene has been identified on chromosome 17. [provided by RefSeq, May 2014]
Uniprot Description
gp130: gp130 is a ubiquitously expressed type I cytokine family receptor. The receptor systems for IL6, LIF, OSM, CNTF, IL11 AND CT1 utilize gp130 for initiating signal transmission. Binds to IL6/IL6R (alpha chain) complex, resulting in the formation of high-affinity IL6 binding sites, and transduces the signal. Does not directly bind IL6. May have a role in embryonic development. Contains 5 fibronectin type III domains and 1 immunoglobulin-like C2-type domain. Two alternatively spliced isoforms have been described.
Protein type: Membrane protein, integral; Receptor, cytokine
Chromosomal Location of Human Ortholog: 5q11.2
Cellular Component: extracellular space; membrane; cell soma; oncostatin-M receptor complex; dendrite; plasma membrane; extracellular region; interleukin-6 receptor complex; external side of plasma membrane
Molecular Function: interleukin-11 binding; protein binding; interleukin-6 receptor binding; interleukin-6 binding; interleukin-27 receptor activity; protein homodimerization activity; leukemia inhibitory factor receptor activity; oncostatin-M receptor activity; growth factor binding; ciliary neurotrophic factor receptor activity; interleukin-6 receptor activity; ciliary neurotrophic factor receptor binding; interleukin-11 receptor activity
Biological Process: glycogen metabolic process; viral reproduction; leukemia inhibitory factor signaling pathway; cytokine and chemokine mediated signaling pathway; positive regulation of tyrosine phosphorylation of Stat3 protein; positive regulation of osteoblast differentiation; positive regulation of tyrosine phosphorylation of Stat1 protein; regulation of Notch signaling pathway; positive regulation of acute inflammatory response; response to cytokine stimulus; positive regulation of cell proliferation; positive regulation of T cell proliferation; positive regulation of adaptive immune response; positive regulation of astrocyte differentiation; negative regulation of apoptosis
Research Articles on IL-6 sR
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Product Notes
The IL-6 sR il6st (Catalog #AAA590012) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Interleukin-6 Soluble Receptor, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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