Human Interleukin 6 ELISA Kit | IL-6 elisa kit

Human Interleukin 6 ELISA Kit

Cat. Num. AAA760693

• Gene Names: IL6; CDF; HGF; HSF; BSF2; IL-6; BSF-2; IFNB2; IFN-beta-2
• Reactivity: Human
• Synonyms: Interleukin 6; Human Interleukin 6 ELISA Kit; IL-6 (Interleukin 6)/IL6/BSF2/HGF/HSF/IFNB2/CDF; IL-6 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of IL-6. No significant cross-reactivity or interference between IL-6 and analogues was observed.
• Sequence Length: 212

• Samples: Serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 4.688-300pg/ml
• Sensitivity: 2.813pg/ml
• Intra-assay Precision: CV<8%
• Inter-assay Precision: CV<10%
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for IL-6 elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Capture antibody was pre-coated onto 96-well plates. And the biotin conjugated antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of target can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 10834984
• NCBI GeneID: 3569
• NCBI Accession #: NP_000591.1
• NCBI GenBank Nucleotide #: NM_000600.4
• Molecular Weight: 23,718 Da
• NCBI Official Full Name: interleukin-6 isoform 1
• NCBI Official Synonym Full Names: interleukin 6
• NCBI Official Symbol: IL6
• NCBI Official Synonym Symbols: CDF; HGF; HSF; BSF2; IL-6; BSF-2; IFNB2; IFN-beta-2
• NCBI Protein Information: interleukin-6
• UniProt Protein Name: Interleukin-6
• UniProt Gene Name: IL6
• UniProt Synonym Gene Names: IFNB2; IL-6; BSF-2; CDF; IFN-beta-2
• UniProt Entry Name: IL6_HUMAN

NCBI Description

This gene encodes a cytokine that functions in inflammation and the maturation of B cells. In addition, the encoded protein has been shown to be an endogenous pyrogen capable of inducing fever in people with autoimmune diseases or infections. The protein is primarily produced at sites of acute and chronic inflammation, where it is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha. The functioning of this gene is implicated in a wide variety of inflammation-associated disease states, including suspectibility to diabetes mellitus and systemic juvenile rheumatoid arthritis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2015]

Uniprot Description

IL6: Cytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response. Plays an essential role in the final differentiation of B-cells into Ig- secreting cells Involved in lymphocyte and monocyte differentiation. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation Acts on B-cells, T-cells, hepatocytes, hematopoietic progenitor cells and cells of the CNS. Also acts as a myokine. It is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance. Genetic variations in IL6 are associated with susceptibility to rheumatoid arthritis systemic juvenile (RASJ). An inflammatory articular disorder with systemic- onset beginning before the age of 16. It represents a subgroup of juvenile arthritis associated with severe extraarticular features and occasionally fatal complications. During active phases of the disorder, patients display a typical daily spiking fever, an evanescent macular rash, lymphadenopathy, hepatosplenomegaly, serositis, myalgia and arthritis. A IL6 promoter polymorphism is associated with a lifetime risk of development of Kaposi sarcoma in HIV-infected men. Belongs to the IL-6 superfamily.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 7p21

Cellular Component: cytoplasm; external side of plasma membrane; extracellular region; extracellular space; interleukin-6 receptor complex

Molecular Function: cytokine activity; growth factor activity; interleukin-6 receptor binding; protein binding

Biological Process: activation of NF-kappaB transcription factor; acute-phase response; aging; bone remodeling; cell growth; cell redox homeostasis; cytokine and chemokine mediated signaling pathway; defense response to Gram-negative bacterium; defense response to Gram-positive bacterium; defense response to protozoan; defense response to virus; endocrine pancreas development; glucose homeostasis; hepatic immune response; humoral immune response; inflammatory response; monocyte chemotaxis; muscle maintenance; negative regulation of apoptosis; negative regulation of caspase activity; negative regulation of cell proliferation; negative regulation of chemokine biosynthetic process; negative regulation of collagen biosynthetic process; negative regulation of cytokine secretion; negative regulation of fat cell differentiation; negative regulation of gluconeogenesis; negative regulation of hormone secretion; negative regulation of muscle development; negative regulation of protein kinase activity; neurite development; neutrophil apoptosis; neutrophil mediated immunity; platelet activation; positive regulation of acute inflammatory response; positive regulation of apoptosis; positive regulation of B cell activation; positive regulation of cell proliferation; positive regulation of chemokine production; positive regulation of DNA replication; positive regulation of epithelial cell proliferation; positive regulation of immunoglobulin secretion; positive regulation of interleukin-6 production; positive regulation of JAK-STAT cascade; positive regulation of leukocyte chemotaxis; positive regulation of MAPKKK cascade; positive regulation of neuron differentiation; positive regulation of nitric oxide biosynthetic process; positive regulation of osteoblast differentiation; positive regulation of peptidyl-serine phosphorylation; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of protein import into nucleus, translocation; positive regulation of protein kinase B signaling cascade; positive regulation of smooth muscle cell proliferation; positive regulation of T cell proliferation; positive regulation of T-helper 2 cell differentiation; positive regulation of transcription factor activity; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; positive regulation of translation; positive regulation of transmission of nerve impulse; positive regulation of tyrosine phosphorylation of Stat3 protein; regulation of angiogenesis; regulation of cell shape; regulation of circadian sleep/wake cycle, non-REM sleep; response to amino acid stimulus; response to antibiotic; response to caffeine; response to calcium ion; response to cold; response to drug; response to electrical stimulus; response to glucocorticoid stimulus; response to heat; response to insulin stimulus; response to nutrient levels; response to peptidoglycan; response to yeast

Disease: Arteriovenous Malformations Of The Brain; Inflammatory Bowel Disease 1; Kaposi Sarcoma, Susceptibility To; Rheumatoid Arthritis, Systemic Juvenile