Canine Interleukin 22 ELISA Kit | IL-22 elisa kit

Canine Interleukin 22 ELISA Kit

Cat. Num. AAA44743

• Gene Names: IL22; TIFa; IL-21; IL-22; ILTIF; IL-TIF; IL-D110; zcyto18; TIFIL-23
• Reactivity: Canine
• Synonyms: Interleukin 22; Canine Interleukin 22 ELISA Kit; IL-22 elisa kit

• Reactivity: Canine
• Specificity: No significant cross-reactivity or interference between Canine IL-22 and analogues was observed.

• Samples: Serum, Plasma, Tissue Homogenate, Feces and Urine
• Assay Type: Sandwich
• Detection Range: 31.2pg/ml-1000pg/ml.
• Sensitivity: 5.0pg/ml.
• Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic applications! It is intended to be determinated IL-22 concentrations in Canine serum, plasma and other body fluids. Using Purified Canine IL-22 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add IL-22 and IL-22 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of IL-22 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• Intra-assay Precision: Intra-assay CV (%) is less than 15%.
• Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]
• Preparation and Storage: Store all reagents at 2-8 degree C

Related Product Information for IL-22 elisa kit

Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only! It is intended to be determinated IL-22 concentrations in Canine serum, plasma and other body fluids. Using Purified Canine IL-22 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add IL-22 and IL-22 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of IL-22 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 10092625
• NCBI GeneID: 50616
• NCBI Accession #: NP_065386.1
• NCBI GenBank Nucleotide #: NM_020525.4
• UniProt Accession #: Q9GZX6
• Molecular Weight: 20,011 Da
• NCBI Official Full Name: interleukin-22
• NCBI Official Synonym Full Names: interleukin 22
• NCBI Official Symbol: IL22
• NCBI Official Synonym Symbols: TIFa; IL-21; IL-22; ILTIF; IL-TIF; IL-D110; zcyto18; TIFIL-23
• NCBI Protein Information: interleukin-22; cytokine Zcyto18; IL-10-related T-cell-derived inducible factor; IL-10-related T-cell-derived-inducible factor
• UniProt Protein Name: Interleukin-22
• UniProt Gene Name: IL22
• UniProt Synonym Gene Names: ILTIF; ZCYTO18; IL-22; IL-TIF
• UniProt Entry Name: IL22_HUMAN

Uniprot Description

IL22: Cytokine that contributes to the inflammatory response in vivo. Belongs to the IL-10 family.

Protein type: Cytokine; Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 12q15

Cellular Component: extracellular space; extracellular region

Molecular Function: interleukin-22 receptor binding; cytokine activity

Biological Process: cell-cell signaling; response to glucocorticoid stimulus; acute-phase response; inflammatory response