Typical Testing Data/Standard Curve (for reference only)
Rat Anti-Interleukin 22 antibody ELISA Kit | IL-22 Ab elisa kit
Rat Anti-Interleukin 22 antibody ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: This ADM enzyme linked immunosorbent assay applies a technique called a quantitative sandwich immunoassay. The microtiter plate provided in this kit has been pre-coated with a monoclonal antibody specific for ADM. Standards or samples are then added to the microtiter plate wells and ADM if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of ADM present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for ADM are added to each well to "sandwich" the ADM immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, A and B substrate solution is added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period.Only those wells that contain ADM and enzyme-conjugated antibody will exhibit a change in colour. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the colour change is measured spectrophotometrically at a wavelength of 450 nm.
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a preprohormone which is cleaved to form two biologically active peptides, adrenomedullin and proadrenomedullin N-terminal 20 peptide. Adrenomedullin is a 52 aa peptide with several functions, including vasodilation, regulation of hormone secretion, promotion of angiogenesis, and antimicrobial activity. The antimicrobial activity is antibacterial, as the peptide has been shown to kill E. coli and S. aureus at low concentration. [provided by RefSeq, Aug 2014]
Uniprot Description
ADM: AM and PAMP are potent hypotensive and vasodilatator agents. Numerous actions have been reported most related to the physiologic control of fluid and electrolyte homeostasis. In the kidney, am is diuretic and natriuretic, and both am and pamp inhibit aldosterone secretion by direct adrenal actions. In pituitary gland, both peptides at physiologically relevant doses inhibit basal ACTH secretion. Both peptides appear to act in brain and pituitary gland to facilitate the loss of plasma volume, actions which complement their hypotensive effects in blood vessels. Belongs to the adrenomedullin family.
Protein type: Secreted; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 11p15.4
Cellular Component: extracellular space; cytoplasm; extracellular region
Molecular Function: protein binding; adrenomedullin receptor binding; hormone activity; receptor binding
Biological Process: developmental growth; blood circulation; positive regulation of apoptosis; heart development; response to glucocorticoid stimulus; response to lipopolysaccharide; female pregnancy; signal transduction; positive regulation of vasodilation; response to insulin stimulus; negative regulation of cell proliferation; elevation of cytosolic calcium ion concentration; cAMP-mediated signaling; cell-cell signaling; receptor internalization; positive regulation of cAMP biosynthetic process; positive regulation of cell proliferation; response to wounding; vasculogenesis; regulation of the force of heart contraction; aging; hormone secretion; progesterone biosynthetic process; organ regeneration; androgen metabolic process; neurite regeneration; positive regulation of heart rate; negative regulation of vasoconstriction; odontogenesis of dentine-containing teeth; response to starvation; positive regulation of angiogenesis; G-protein coupled receptor internalization; neural tube closure; negative regulation of vascular permeability; response to hypoxia; cAMP biosynthetic process; response to cold
Research Articles on IL-22 Ab
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Product Notes
The Rat IL-22 Ab adm (Catalog #AAA727196) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA727196 ELISA Kit recognizes Rat IL-22 Ab. It is sometimes possible for the material contained within the vial of "Anti-Interleukin 22 antibody, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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