IL-18 recombinant protein

Human IL-18 Protein

Cat. Num. AAA9718667

• Gene Names: IL18; IGIF; IL-18; IL-1g; IL1F4
• Purity: >85% as determined by SDS-PAGE
• Synonyms: IL-18; Human IL-18 Protein; Recombinant Human IL18 Protein (Interleukin-18) expressed in E. coli; IGIF; IL-1g; IL1F4; Interleukin 18; Interleukin-18; IL18; IL-18 recombinant protein

• Host: E Coli
• Purity/Purification: >85% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH 7.4.
• Sequence: Amino acid sequence derived from human IL18 (NP_001553.1) (Tyr37-Asp193) was expressed.
• Sequence Length: 193

• Species: Human
• Protein Length: The recombinant human IL18 consists 157 amino acids and predicts a molecular mass of 18.2 kDa.
• Usage Notes: Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized Human IL18 protein in sterile ddH2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
• Preparation and Storage: Item should be stored desiccated below -20 degree C. Upon reconstitution, the protein should be stored at 4 degree C between 2-7 days and for future use below -20 degree C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

SDS-Page

(Fig.SDS-PAGE analysis of Human IL-18 protein.)

Related Product Information for IL-18 recombinant protein

Interleukin-18 (IL-18, also known as interferon-gamma inducing factor) is a proinflammatory cytokine that belongs to the IL-1 superfamily and is produced by macrophages and other cells. This cytokine can induce the IFN-gamma production of T cells. The combination of IL-18 and IL12 has been shown to inhibit IL4 dependent IgE and IgG1 production, and enhance IgG2a production of B cells. IL-18 binding protein (IL18BP) can specifically interact with this cytokine, and thus negatively regulate its biological activity. IL-18 is an IL-1-like cytokine that requires cleavage with caspase-1 to become active, was found to increase IgE production in a CD4+ T cells-, IL-4- and STAT6-dependent fashion. IL-18 and T cell receptor-mediated stimulation could induce naïve CD4+ T cells to develop into IL-4-producing cells in vitro. Thus, caspase-1 and IL-18 may be critical in regulation of IgE production in vivo, providing a potential therapeutic target for allergic disorders. IL-18 production in primary synovial cultures and purified synovial fibroblasts was, in turn, upregulated by TNF-alpha and IL-1beta, suggesting that monokine expression can feed back to promote Th1 cell development in synovial membrane. Besides, synergistic combinations of IL-18, IL-12, and IL-15 may be of importance in sustaining both Th1 responses and monokine production in RA.

NCBI and Uniprot Product Information

• NCBI GI #: 4504653
• NCBI GeneID: 3606
• NCBI Accession #: NP_001553.1
• NCBI GenBank Nucleotide #: NP_001553.1
• UniProt Accession #: Q14116
• Molecular Weight: 18.2 kDa
• NCBI Official Full Name: interleukin-18 isoform 1
• NCBI Official Synonym Full Names: interleukin 18
• NCBI Official Symbol: IL18
• NCBI Official Synonym Symbols: IGIF; IL-18; IL-1g; IL1F4
• NCBI Protein Information: interleukin-18

NCBI Description

The protein encoded by this gene is a proinflammatory cytokine that augments natural killer cell activity in spleen cells, and stimulates interferon gamma production in T-helper type I cells. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Aug 2011]