Typical Testing Data/Standard Curve (for reference only)
Sheep Interleukin 11 ELISA Kit | IL-11 elisa kit
Sheep Interleukin 11 ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: IL-11 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for IL-11. Standards or samples are then added to the microtiter plate wells and IL-11 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of IL-11 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for IL-11 are added to each well to "sandwich" the IL-11 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain IL-11 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IL-11 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a member of the gp130 family of cytokines. These cytokines drive the assembly of multisubunit receptor complexes, all of which contain at least one molecule of the transmembrane signaling receptor IL6ST (gp130). This cytokine is shown to stimulate the T-cell-dependent development of immunoglobulin-producing B cells. It is also found to support the proliferation of hematopoietic stem cells and megakaryocyte progenitor cells. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jun 2012]
Uniprot Description
IL11: Directly stimulates the proliferation of hematopoietic stem cells and megakaryocyte progenitor cells and induces megakaryocyte maturation resulting in increased platelet production. Belongs to the IL-6 superfamily.
Protein type: Motility/polarity/chemotaxis; Cell cycle regulation; Cytokine; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 19q13.3-q13.4
Cellular Component: extracellular space; cytoplasm; extracellular region
Molecular Function: growth factor activity; cytokine activity; interleukin-11 receptor binding
Biological Process: fat cell differentiation; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of MAPKKK cascade; cell-cell signaling; megakaryocyte differentiation; B cell differentiation; negative regulation of hormone secretion; positive regulation of cell proliferation; positive regulation of transcription from RNA polymerase II promoter; positive regulation of peptidyl-serine phosphorylation
Research Articles on IL-11
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Product Notes
The Sheep IL-11 il11 (Catalog #AAA735049) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA735049 ELISA Kit recognizes Sheep IL-11. It is sometimes possible for the material contained within the vial of "Interleukin 11, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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