Porcine Inhibitor k Binding ELISA Kit | IKB elisa kit

Porcine Inhibitor k Binding ELISA Kit

Cat. Num. AAA738338

• Gene Names: NFKBIA; IKBA; MAD-3; NFKBI
• Reactivity: Porcine
• Synonyms: Inhibitor k Binding; Porcine Inhibitor k Binding ELISA Kit; IKB elisa kit

• Reactivity: Porcine

• Samples: Serum, plasma, cell culture superna tants, body fluid and tissue homogenate
• Assay Type: Competitive
• Sensitivity: 0.1 ng/mL.
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for IKB elisa kit

For samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

INTENDED USE This IKB ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Porcine IKB. This ELISA kit for research use only!

PRINCIPLE OF THE ASSAY IKB ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-IKB antibody and an IKB-HRP conjugate. The assay sample and buffer are incubated together with IKB-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the IKB concentration since IKB from samples and IKB-HRP conjugate compete for the anti-IKB antibody binding site. Since the number of sites is limited, as more sites are occupied by IKB from the sample, fewer sites are left to bind IKB-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IKB concentration in each sample is interpolated from this standard curve.

Product Categories/Family for IKB elisa kit

Cell Biology

NCBI and Uniprot Product Information

• NCBI GI #: 6706211
• NCBI GeneID: 4792
• UniProt Accession #: P25963; B2R8L6
• Molecular Weight: 35,609 Da
• NCBI Official Full Name: IkBa
• NCBI Official Synonym Full Names: nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha
• NCBI Official Symbol: NFKBIA
• NCBI Official Synonym Symbols: IKBA; MAD-3; NFKBI
• NCBI Protein Information: NF-kappa-B inhibitor alpha; ikB-alpha; IkappaBalpha; I-kappa-B-alpha; nuclear factor of kappa light chain gene enhancer in B-cells; major histocompatibility complex enhancer-binding protein MAD3
• UniProt Protein Name: NF-kappa-B inhibitor alpha
• UniProt Gene Name: NFKBIA
• UniProt Synonym Gene Names: IKBA; MAD3; NFKBI; IkB-alpha; IkappaBalpha
• UniProt Entry Name: IKBA_HUMAN

NCBI Description

This gene encodes a member of the NF-kappa-B inhibitor family, which contain multiple ankrin repeat domains. The encoded protein interacts with REL dimers to inhibit NF-kappa-B/REL complexes which are involved in inflammatory responses. The encoded protein moves between the cytoplasm and the nucleus via a nuclear localization signal and CRM1-mediated nuclear export. Mutations in this gene have been found in ectodermal dysplasia anhidrotic with T-cell immunodeficiency autosomal dominant disease. [provided by RefSeq, Aug 2011]

Uniprot Description

IkB-alpha: a regulatory protein that inhibits NF-kappa-B by complexing with and trapping it in the cytoplasm. May be involved in regulation of transcriptional responses to NF-kappa-B, including cell adhesion, immune and proinflammatory responses, apoptosis, differentiation and growth. Controlled by sequential serine-phosphorylation, ubiquitination and degradation.

Protein type: Inhibitor; DNA-binding

Chromosomal Location of Human Ortholog: 14q13

Cellular Component: cytoplasm; plasma membrane; nucleus; cytosol

Molecular Function: NF-kappaB binding; identical protein binding; protein binding; enzyme binding; heat shock protein binding; ubiquitin protein ligase binding; nuclear localization sequence binding; protein complex binding; transcription factor binding

Biological Process: viral reproduction; nerve growth factor receptor signaling pathway; apoptosis; positive regulation of cellular protein metabolic process; toll-like receptor 3 signaling pathway; T cell receptor signaling pathway; toll-like receptor 10 signaling pathway; activation of NF-kappaB transcription factor; toll-like receptor 5 signaling pathway; response to exogenous dsRNA; cytoplasmic sequestering of NF-kappaB; lipopolysaccharide-mediated signaling pathway; positive regulation of interferon type I production; toll-like receptor 4 signaling pathway; cytoplasmic sequestering of transcription factor; negative regulation of myeloid cell differentiation; MyD88-independent toll-like receptor signaling pathway; negative regulation of DNA binding; regulation of NF-kappaB import into nucleus; regulation of cell proliferation; toll-like receptor 2 signaling pathway; MyD88-dependent toll-like receptor signaling pathway; response to muramyl dipeptide; protein import into nucleus, translocation; inhibition of NF-kappaB transcription factor; toll-like receptor signaling pathway; innate immune response; negative regulation of Notch signaling pathway; positive regulation of transcription from RNA polymerase II promoter; toll-like receptor 9 signaling pathway; negative regulation of apoptosis

Disease: Ectodermal Dysplasia, Anhidrotic, With T-cell Immunodeficiency, Autosomal Dominant