Hedgehog Homolog, Indian (IHH) Recombinant Protein | IHH recombinant protein

Recombinant Hedgehog Homolog, Indian (IHH)

Cat. Num. AAA2012372

• Gene Names: IHH; BDA1; HHG2
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Hedgehog Homolog; Indian (IHH); Recombinant Hedgehog Homolog; IHH recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with N-terminal His-Tag, its sequence is listed below.; MGHHHHHHSGSEF-F SDVLIFLDRE PHRLRAFQVI ETQDPPRRLA LTPAHLLFTA DNHTEPAARF RATFASHVQP GQYVLVAGVP GLQPARVAAV STHVALGAYA PLTKHGTLVV EDVVASCFAA VADHHLAQLA FWPLRLFHSL AWGSWTPGEG VHWYPQLLYR LGRLLLEEG
• Sequence Length: 411

• Applicable Applications for IHH recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Homo sapiens (Human)
• Expression System: Prokaryotic expression
• Residues: Phe240~Gly399 (Accession # Q14623) with N-terminal His-Tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 119392086
• NCBI GeneID: 3549
• NCBI Accession #: NP_002172.2
• NCBI GenBank Nucleotide #: NM_002181.3
• UniProt Accession #: Q14623; O43322; Q8N4B9; B9EGM5
• Molecular Weight: 19.2kDa
• NCBI Official Full Name: indian hedgehog protein
• NCBI Official Synonym Full Names: indian hedgehog
• NCBI Official Symbol: IHH
• NCBI Official Synonym Symbols: BDA1; HHG2
• NCBI Protein Information: indian hedgehog protein; HHG-2; Indian hedgehog homolog
• UniProt Protein Name: Indian hedgehog protein
• Protein Family: Indian hedgehog protein
• UniProt Gene Name: IHH
• UniProt Synonym Gene Names: IHH
• UniProt Entry Name: IHH_HUMAN

NCBI Description

This gene encodes a member of the hedgehog family of secreted signaling molecules. Hedgehog proteins are essential regulators of a variety of developmental processes including growth, patterning and morphogenesis. The encoded protein specifically plays a role in bone growth an differentiation. Mutations in this gene are the cause of brachydactyly type A1 which is characterized by shortening or malformation of the phalanges. Mutations in this gene are also the cause of acrocapitofemoral dysplasia. [provided by RefSeq, Feb 2010]

Uniprot Description

IHH: Intercellular signal essential for a variety of patterning events during development. Binds to the patched (PTC) receptor, which functions in association with smoothened (SMO), to activate the transcription of target genes. Implicated in endochondral ossification: may regulate the balance between growth and ossification of the developing bones. Induces the expression of parathyroid hormone-related protein (PTHRP). Expressed in embryonic lung, and in adult kidney and liver. Belongs to the hedgehog family.

Chromosomal Location of Human Ortholog: 2q33-q35

Cellular Component: extracellular matrix; extracellular space; plasma membrane

Molecular Function: peptidase activity; patched binding; calcium ion binding

Biological Process: vitelline membrane formation; proteoglycan metabolic process; maternal process involved in pregnancy; positive regulation of collagen biosynthetic process; cell maturation; cell fate specification; proteolysis; embryonic pattern specification; response to estradiol stimulus; positive regulation of T cell differentiation in the thymus; smooth muscle development; cell-cell signaling; embryonic digestive tract morphogenesis; regulation of growth; positive regulation of mesenchymal cell proliferation; pancreas development; positive regulation of smoothened signaling pathway; heart looping; skeletal development; bone resorption; negative regulation of immature T cell proliferation in the thymus; smoothened signaling pathway; intein-mediated protein splicing; in utero embryonic development; multicellular organism growth; negative regulation of eye pigmentation; negative regulation of T cell differentiation in the thymus; negative regulation of signal transduction; osteoblast differentiation; patterning of blood vessels; positive regulation of chondrocyte differentiation; cartilage development; neuron development; positive regulation of transcription from RNA polymerase II promoter; embryonic camera-type eye morphogenesis; embryonic digit morphogenesis; positive regulation of alpha-beta T cell differentiation; positive regulation of epithelial cell proliferation; negative regulation of alpha-beta T cell differentiation; negative regulation of apoptosis

Disease: Brachydactyly, Type A1; Acrocapitofemoral Dysplasia