IGSF8 recombinant protein

IGSF8 Protein, Human, Recombinant (His Tag)

Cat. Num. AAA8121409

• Gene Names: IGSF8; EWI2; PGRL; CD316; EWI-2; KCT-4; CD81P3; LIR-D1
• Purity: >95% as determined by SDS-PAGE
• Synonyms: IGSF8; IGSF8 Protein; Human; Recombinant (His Tag); Human PGRL/IGSF8 Protein (His Tag); CD316 Protein; CD81P3 Protein; EWI-2 Protein; EWI2 Protein; KCT-4 Protein; LIR-D1 Protein; PGRL Protein; immunoglobulin superfamily; member 8; IGSF8 recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Thr579

• Species: Human
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Arg 28
• Tag: C-His
• Protein Construction: A DNA sequence encoding the human IGSF8 (Q969P0-1)(Met1-Thr579) was expressed with a polyhistidine tag at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

References

Glazar AI, et al. (2009) Immunoglobulin superfamily member IgSF8 (EWI-2) and CD9 in fertilisation: evidence of distinct functions for CD9 and a CD9-associated protein in mammalian sperm-egg interaction. Reprod Fertil Dev. 21(2): 293-303.Murdoch JN, et al. (2003) Genomic organization and embryonic expression of Igsf8, an immunoglobulin superfamily member implicated in development of the nervous system and organ epithelia. Mol Cell Neurosci. 22(1): 62-74.Zhang XA, et al. (2003) EWI2/PGRL associates with the metastasis suppressor KAI1/CD82 and inhibits the migration of prostate cancer cells. Cancer Res. 63 (10): 2665-74.

NCBI and Uniprot Product Information

• NCBI GI #: 16445029
• NCBI GeneID: 93185
• NCBI Accession #: NP_443100.1
• NCBI GenBank Nucleotide #: NM_052868.6
• UniProt Accession #: Q969P0; Q8NG09; Q96DP4; Q9BTG9
• Molecular Weight: 40,191 Da
• NCBI Official Full Name: immunoglobulin superfamily member 8
• NCBI Official Synonym Full Names: immunoglobulin superfamily member 8
• NCBI Official Symbol: IGSF8
• NCBI Official Synonym Symbols: EWI2; PGRL; CD316; EWI-2; KCT-4; CD81P3; LIR-D1
• NCBI Protein Information: immunoglobulin superfamily member 8
• UniProt Protein Name: Immunoglobulin superfamily member 8
• Protein Family: Immunoglobulin superfamily
• UniProt Gene Name: IGSF8
• UniProt Synonym Gene Names: CD81P3; EWI2; KCT4; IgSF8; EWI-2; KCT-4; PGRL

NCBI Description

This gene encodes a member the EWI subfamily of the immunoglobulin protein superfamily. Members of this family contain a single transmembrane domain, an EWI (Glu-Trp-Ile)-motif and a variable number of immunoglobulin domains. This protein interacts with the tetraspanins CD81 and CD9 and may regulate their role in certain cellular functions including cell migration and viral infection. The encoded protein may also function as a tumor suppressor by inhibiting the proliferation of certain cancers. Alternate splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq, Sep 2011]

Uniprot Description

May play a key role in diverse functions ascribed to CD81 and CD9 such as oocytes fertilization or hepatitis C virus function. May regulate proliferation and differentiation of keratinocytes. May be a negative regulator of cell motility: suppresses T-cell mobility coordinately with CD81, associates with CD82 to suppress prostate cancer cell migration, regulates epidermoid cell reaggregation and motility on laminin-5 with CD9 and CD81 as key linkers. May also play a role on integrin-dependent morphology and motility functions. May participate in the regulation of neurite outgrowth and maintenance of the neural network in the adult brain.