IgG4-Fc recombinant protein

Recombinant Human IgG4-Fc Protein

Cat. Num. AAA2557259

• Purity: >95% as determined by reducing SDS-PAGE.
• Synonyms: IgG4-Fc; Recombinant Human IgG4-Fc Protein; Ig gamma-4 chain C region; IgG4 Fc; IgG4-Fc recombinant protein

• Host: Human Cells
• Purity/Purification: >95% as determined by reducing SDS-PAGE.
• Form/Format: Lyophilized from a 0.2 mum filtered solution of 20mM PB, 150mM NaCl, pH7.4.
• Sequence: Glu99-Gly326
• Sequence Length: 327

• Species: Human
• Endotoxin: < 1.0 EU per mug as determined by the LAL method.
• Preparation and Storage: Lyophilized protein should be stored at < -20 degree C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7 degree C for 2-7 days. Aliquots of reconstituted samples are stable at < -20 degree C for 3 months.

Related Product Information for IgG4-Fc recombinant protein

Protein Construction: Recombinant Human IgG4-Fc is produced by our mammalian expression system and the target gene encoding Glu99-Gly326 is expressed.

Background: As a monomeric immunoglobulin that is predominately involved in the secondary antibody response and the only isotype that can pass through the human placenta, Immunoglobulin G (IgG) is synthesized and secreted by plasma B cells, and constitutes 75% of serum immunoglobulins in humans. IgG antibodies protect the body against the pathogens by agglutination and immobilization, complement activation, toxin neutralization, as well as the antibody-dependent cell-mediated cytotoxicity (ADCC). IgG tetramer contains two heavy chains (50 kDa) and two light chains (25 kDa) linked by disulfide bonds, that is the two identical halves form the Y-like shape. IgG is digested by pepsin proteolysis into Fab fragment (antigen-binding fragment) and Fc fragment ("crystallizable" fragment). IgG1 is most abundant in serum among the four IgG subclasses (IgG1, 2, 3 and 4) and binds to Fc receptors (FcgammaR) on phagocytic cells with high affinity. Fc fragment is demonstrated to mediate phagocytosis, trigger inflammation, and target Ig to particular tissues. Protein G or Protein A on the surface of certain Staphylococcal and Streptococcal strains specifically binds with the Fc region of IgGs, and has numerous applications in biotechnology as a reagent for affinity purification. Recombinant IgG Fc Region is suggested to represent a potential anti-inflammatory drug for treatment of human autoimmune diseases.

NCBI and Uniprot Product Information

• NCBI GI #: 121047
• NCBI GeneID: 3503
• UniProt Accession #: P01861
• Molecular Weight: Molecular Mass: 25.6kDa; Actual Protein Molecular Mass: 32kDa
• NCBI Official Full Name: Immunoglobulin heavy constant gamma 4
• NCBI Official Synonym Full Names: immunoglobulin heavy constant gamma 4 (G4m marker)
• NCBI Official Symbol: IGHG4
• UniProt Protein Name: Ig gamma-4 chain C region
• UniProt Gene Name: IGHG4
• UniProt Entry Name: IGHG4_HUMAN

Uniprot Description

IGHG4:

Chromosomal Location of Human Ortholog: 14q32.33

Cellular Component: extracellular space; extracellular region

Molecular Function: antigen binding

Biological Process: innate immune response; complement activation, classical pathway; complement activation