IGFBP6, Recombinant, Mouse, Western Blot Positive Control Recombinant Protein | IGFBP6 recombinant protein
IGFBP6, Recombinant, Mouse, Western Blot Positive Control (Insulin-like Growth Factor-binding Protein 6, IBP-6, IGF-binding Protein 6, IGFBP-6, IBP6)
Gene Names
IGFBP6; IBP6
Applications
Western Blot
Purity
Purified (Highly Purified (~95%))
Synonyms
IGFBP6; Recombinant; Mouse; Western Blot Positive Control; Western Blot Positive Control (Insulin-like Growth Factor-binding Protein 6; IBP-6; IGF-binding Protein 6; IGFBP-6; IBP6); Insulin-like Growth Factor-binding Protein 6; IBP6; IGFBP6 recombinant protein
Purity/Purification
Purified (Highly Purified (~95%))
Form/Format
Supplied as a liquid in SDS-PAGE sample buffer (reduced).
Applicable Applications for IGFBP6 recombinant protein
Western Blot
Application Notes
Suitable for use in Western Blot. Not suitable for use in ELISA.
Recommended Dilutions: Western Blot: 10ul/lane. SDS may crystallize in cold conditions. It should redissolve by warming before taking it from the stock. It should be heated once prior to loading on gels. If the product has been stored for several weeks, then it may be preferable to add 5ul of fresh 2x sample buffer per 10ul of the Western Blot solution prior to heating and loading on gels.
Recommended Dilutions: Western Blot: 10ul/lane. SDS may crystallize in cold conditions. It should redissolve by warming before taking it from the stock. It should be heated once prior to loading on gels. If the product has been stored for several weeks, then it may be preferable to add 5ul of fresh 2x sample buffer per 10ul of the Western Blot solution prior to heating and loading on gels.
Source Information
Recombinant mouse IGFBP6 (24-238aa), expressed in NS0 cells as human CD33 fusion protein.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Shipping Temperature: Blue Ice
Shipping Temperature: Blue Ice
Related Product Information for IGFBP6 recombinant protein
The insulin like growth factors (IGFs) are the major growth-promoting factors in the plasma. IGFs are secreted by a variety of cells and exert a multitude of effects on cellular survival, growth and differentiation. The A and B domains of IGFs are identical to insulin. IGF initiates their biological action through binding to the type IGF receptor (IGF-1R), a heterotrimeric protein complex with a tyrosine kinase activity. The IGF-IIR lacks the kinase activity and is actually identical to the mannose- 6-phophate receptor. Unlike most other peptide hormones, IGFs are complexed with specific binding proteins in the plasma known IGF Binding proteins (IGFBPs). At least 6 related IGFBPs (IGFBP1-6) have been well characterized. Recently, IGFBP-7/Mac25/prostacyclin- stimulating factor (PSF)/tumor adhesion factor (TAF) was originally identified as a cDNA derived from leptomeninges. These proteins are present in plasma in high concentration as compared to the membrane IGFRs. Therefore, IGFBPs have the potential to modulate the IGF action. IGFBPs have been shown to either inhibit or stimulate the IGF effects. The primary structures of mammalian IGFBPs appear to contain three distinct domains of roughly equivalent sizes: the conserved N-terminal domain, the highly variable mid region, and the conserved C-terminal domain. Human IGFBPs share approximately 36% identity.
Human IGFBP-6 is a 240aa (mature protein 27-240aa). IGFBP6 plays a role in lipoprotein assembly and dietary cholesterol absorption. in addition to its acyltransferase activity, it may act as a ligase. may provide cholesteryl esters for lipoprotein secretion from hepatocytes and intestinal mucosa.
Human IGFBP-6 is a 240aa (mature protein 27-240aa). IGFBP6 plays a role in lipoprotein assembly and dietary cholesterol absorption. in addition to its acyltransferase activity, it may act as a ligase. may provide cholesteryl esters for lipoprotein secretion from hepatocytes and intestinal mucosa.
Product Categories/Family for IGFBP6 recombinant protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
~23kD
NCBI Official Full Name
insulin-like growth factor-binding protein 6
NCBI Official Synonym Full Names
insulin-like growth factor binding protein 6
NCBI Official Symbol
IGFBP6
NCBI Official Synonym Symbols
IBP6
NCBI Protein Information
insulin-like growth factor-binding protein 6; IBP-6; IGFBP-6; IGF binding protein 6; IGF-binding protein 6
UniProt Protein Name
Insulin-like growth factor-binding protein 6
Protein Family
UniProt Gene Name
IGFBP6
UniProt Synonym Gene Names
IBP6
UniProt Entry Name
IBP6_HUMAN
Similar Products
Product Notes
The IGFBP6 igfbp6 (Catalog #AAA650394) is a Recombinant Protein and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's IGFBP6, Recombinant, Mouse, Western Blot Positive Control can be used in a range of immunoassay formats including, but not limited to, Western Blot. Suitable for use in Western Blot. Not suitable for use in ELISA. Recommended Dilutions: Western Blot: 10ul/lane. SDS may crystallize in cold conditions. It should redissolve by warming before taking it from the stock. It should be heated once prior to loading on gels. If the product has been stored for several weeks, then it may be preferable to add 5ul of fresh 2x sample buffer per 10ul of the Western Blot solution prior to heating and loading on gels. Researchers should empirically determine the suitability of the IGFBP6 igfbp6 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IGFBP6, Recombinant, Mouse, Western Blot Positive Control, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
