Human ICAM-2 ELISA Kit | ICAM2 elisa kit

Human ICAM-2 PicoKine ELISA Kit

Cat. Num. AAA177268

• Gene Names: ICAM2; CD102
• Reactivity: Human
• Synonyms: ICAM-2; Human ICAM-2 PicoKine ELISA Kit; Intercellular adhesion molecule 2; intercellular adhesion molecule 2; ARNT-interacting protein; Basic-helix-loop-helix-PAS protein MOP1; BB2; bHLHe78; CD54; Class E basic helix-loop-helix protein 78; HIF1; HIF1-alpha; Hypoxia inducible factor 1; alpha subunit (basic helix-loop-helix transcription factor); Hypoxia-inducible factor 1-alpha; Intercellular adhesion molecule 1; Major group rhinovirus receptor; Member of PAS protein 1; MOP1; P3.58; PAS domain-containing protein 8; PASD8; ICAM2 elisa kit

• Reactivity: Human
• Specificity: Natural and recombinant Human ICAM2
• Sequence Length: 275

• Samples: Cell culture supernates, serum and plasma(heparin, edta)
• Detection Range: 312pg/ml-20,000pg/ml
• Sensitivity: <10pg/ml
• Intra-assay Precision: Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested on one plate to assess intra-assay precision.
• Inter-assay Precision: Inter-Assay Precision (Precision across assays):Three samples of known concentration were tested in separate assays to assess interassay precision.
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ICAM2 elisa kit

Principle of the Assay: The Picokine™ Human ICAM2 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Human ICAM2 with a 96-well strip plate that is pre-coated with antibody specific for ICAM2. The detection antibody is a biotinylated antibody specific for ICAM2. The capture antibody is monoclonal antibody from mouse, the detection antibody is polyclonal antibody from goat. The kit contains recombinant Human ICAM2 with immunogen: Expression system for standard: NSO; Immunogen sequence: K25-Q223. The kit is analytically validated with ready to use reagents. To measure Human ICAM2, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly proportional to Human ICAM2 in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Human ICAM2 in the sample.

Background/Introduction: Intercellular adhesion molecule 2(ICAM2), also known as CD102(Cluster of Differentiation 102) is an integral membrane protein that has 2 immunoglobulin-like domains. This gene is mapped to 17q23.3. The protein encoded by this gene is a member of the intercellular adhesion molecule(ICAM) family. All ICAM proteins are type I transmembrane glycoproteins, contain 2-9 immunoglobulin-like C2-type domains, and bind to the leukocyte adhesion LFA-1 protein. This protein may play a role in lymphocyte recirculation by blocking LFA-1-dependent cell adhesion. It mediates adhesive interactions important for antigen-specific immune response, NK-cell mediated clearance, lymphocyte recirculation, and other cellular interactions important for immune response and surveillance.

NCBI and Uniprot Product Information

• NCBI GI #: 115502404
• NCBI GeneID: 3384
• UniProt Accession #: P13598; Q14600
• Molecular Weight: 30,654 Da
• NCBI Official Full Name: Intercellular adhesion molecule 2
• NCBI Official Synonym Full Names: intercellular adhesion molecule 2
• NCBI Official Symbol: ICAM2
• NCBI Official Synonym Symbols: CD102
• NCBI Protein Information: intercellular adhesion molecule 2; ICAM-2
• UniProt Protein Name: Intercellular adhesion molecule 2
• Protein Family: Intercellular adhesion molecule
• UniProt Gene Name: ICAM2
• UniProt Synonym Gene Names: ICAM-2
• UniProt Entry Name: ICAM2_HUMAN

NCBI Description

The protein encoded by this gene is a member of the intercellular adhesion molecule (ICAM) family. All ICAM proteins are type I transmembrane glycoproteins, contain 2-9 immunoglobulin-like C2-type domains, and bind to the leukocyte adhesion LFA-1 protein. This protein may play a role in lymphocyte recirculation by blocking LFA-1-dependent cell adhesion. It mediates adhesive interactions important for antigen-specific immune response, NK-cell mediated clearance, lymphocyte recirculation, and other cellular interactions important for immune response and surveillance. Several transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

ICAM2: ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). ICAM2 may play a role in lymphocyte recirculation by blocking LFA-1-dependent cell adhesion. It mediates adhesive interactions important for antigen- specific immune response, NK-cell mediated clearance, lymphocyte recirculation, and other cellular interactions important for immune response and surveillance. Belongs to the immunoglobulin superfamily. ICAM family.

Protein type: Cell adhesion; Membrane protein, integral

Chromosomal Location of Human Ortholog: 17q23.3

Cellular Component: membrane; integral to plasma membrane; plasma membrane; uropod

Molecular Function: integrin binding

Biological Process: extracellular matrix organization and biogenesis; cell-cell adhesion; regulation of immune response; cell adhesion