Bovine Bone sialoprotein 2 ELISA Kit | IBSP elisa kit
Bovine Bone sialoprotein 2 (IBSP) ELISA Kit
Reactivity
Bovine
Synonyms
Bone sialoprotein 2; Bovine Bone sialoprotein 2 (IBSP) ELISA Kit; BNSP; BSP; BSP-II; SP-II; bone sialoprotein II; IBSP elisa kit
Reactivity
Bovine
Specificity
This assay has high sensitivity and excellent specificity for detection of Bovine IBSP. No significant cross-reactivity or interference between Bovine IBSP and analogues was observed.
Sequence Length
317
Samples
Serum, Plasma, Other biological fluids
Assay Type
Sandwich
Sample Volume
1-200 uL
Precision
Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV
Detection Wavelength
450 nm
Preparation and Storage
Store at 2-8 degree C.
Related Product Information for IBSP elisa kit
Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate IBSP in samples. An antibody specific for IBSP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyIBSP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IBSP is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IBSP bound in the initial step. The color development is stopped and the intensity of the color is measured.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
34,101 Da
NCBI Official Full Name
bone sialoprotein 2
NCBI Official Synonym Full Names
integrin binding sialoprotein
NCBI Official Symbol
IBSP
NCBI Protein Information
bone sialoprotein 2
UniProt Protein Name
Bone sialoprotein 2
Protein Family
UniProt Gene Name
IBSP
UniProt Synonym Gene Names
BSP II
Uniprot Description
Sialoprotein-2: Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction. Promotes Arg-Gly-Asp-dependent cell attachment.
Protein type: Cell adhesion; Motility/polarity/chemotaxis; Secreted; Secreted, signal peptide
Cellular Component: extracellular matrix; extracellular space; membrane; vesicle
Molecular Function: integrin binding
Biological Process: bone mineralization; cell adhesion; cellular response to growth factor stimulus; extracellular matrix organization; osteoblast differentiation; positive regulation of cell adhesion
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Product Notes
The Bovine IBSP ibsp (Catalog #AAA284500) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA284500 ELISA Kit recognizes Bovine IBSP. It is sometimes possible for the material contained within the vial of "Bone sialoprotein 2, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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