CCR2 sirna

CCR2 siRNA (Human)

Cat. Num. AAA8218422

• Gene Names: CCR2; CKR2; CCR-2; CCR2A; CCR2B; CD192; CKR2A; CKR2B; CMKBR2; MCP-1-R; CC-CKR-2
• Reactivity: Human
• Applications: RNA Interference (RNAi)
• Purity: > 97%
• Synonyms: CCR2; CCR2 siRNA (Human); CMKBR2; C-C chemokine receptor type 2; C-C CKR-2; CC-CKR-2; CCR-2; Monocyte chemoattractant protein 1 receptor; MCP-1-R; CD antigen CD192; CCR2 sirna

• Reactivity: Human
• Specificity: CCR2 siRNA (Human) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
• Purity/Purification: > 97%
• Form/Format: Lyophilized powder
• Sequence Length: 374

• Applicable Applications for CCR2 sirna: RNA Interference (RNAi)
• Source: Synthetic
• Quality Control: Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
• Directions for Use: We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
• Components: We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of human CCR2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
• Preparation and Storage: Shipped at 4 degree C. Store at -20 degree C for one year.

Related Product Information for CCR2 sirna

siRNA to inhibit CCR2 expression using RNA interference

NCBI and Uniprot Product Information

• NCBI GI #: 183979980
• NCBI GeneID: 729230
• NCBI Accession #: NP_001116513.2
• NCBI GenBank Nucleotide #: NM_001123041.2
• UniProt Accession #: P41597; Q4VBL2; A0AVQ3; B2RMT0
• Molecular Weight: 41,064 Da
• NCBI Official Full Name: C-C chemokine receptor type 2 isoform A
• NCBI Official Synonym Full Names: chemokine (C-C motif) receptor 2
• NCBI Official Symbol: CCR2
• NCBI Official Synonym Symbols: CKR2; CCR-2; CCR2A; CCR2B; CD192; CKR2A; CKR2B; CMKBR2; MCP-1-R; CC-CKR-2
• NCBI Protein Information: C-C chemokine receptor type 2
• UniProt Protein Name: C-C chemokine receptor type 2
• Protein Family: C-C chemokine receptor
• UniProt Gene Name: CCR2
• UniProt Synonym Gene Names: CMKBR2; C-C CKR-2; CC-CKR-2; CCR-2; CCR2; MCP-1-R
• UniProt Entry Name: CCR2_HUMAN

NCBI Description

This gene encodes two isoforms of a receptor for monocyte chemoattractant protein-1, a chemokine which specifically mediates monocyte chemotaxis. Monocyte chemoattractant protein-1 is involved in monocyte infiltration in inflammatory diseases such as rheumatoid arthritis as well as in the inflammatory response against tumors. The receptors encoded by this gene mediate agonist-dependent calcium mobilization and inhibition of adenylyl cyclase. This gene is located in the chemokine receptor gene cluster region. Two alternatively spliced transcript variants are expressed by the gene. [provided by RefSeq, Mar 2009]

Uniprot Description

CCR2: a seven transmembrane protein closely related to CCR1. Receptor for the MCP-1, MCP-3 and MCP-4 chemokines. Expressed at high levels in primary neutrophils and primary monocytes, and is further upregulated on neutrophil activation and during monocyte to macrophage differentiation. Transduces a signal by increasing the intracellular calcium ions level. Alternative co receptor with CD4 for HIV-1 infection. Two splice-variant isoforms have been described.

Protein type: Receptor, GPCR; GPCR, family 1; Membrane protein, multi-pass; Membrane protein, integral

Chromosomal Location of Human Ortholog: 3p21.31

Cellular Component: cell soma; perinuclear region of cytoplasm; integral to plasma membrane; cytoplasm; dendrite; integral to membrane; plasma membrane; perikaryon; cytosol

Molecular Function: protein homodimerization activity; CCR2 chemokine receptor binding; C-C chemokine receptor activity; chemokine receptor activity

Biological Process: positive regulation of T-helper 1 type immune response; viral reproduction; chemotaxis; elevation of cytosolic calcium ion concentration; response to wounding; dendritic cell chemotaxis; cellular homeostasis; inflammatory response; positive regulation of tumor necrosis factor biosynthetic process; cytokine and chemokine mediated signaling pathway; negative regulation of adenylate cyclase activity; JAK-STAT cascade; cellular calcium ion homeostasis; G-protein coupled receptor protein signaling pathway; negative regulation of angiogenesis; positive regulation of interferon-gamma production; negative regulation of T-helper 2 type immune response; positive regulation of interleukin-2 production; innate immune response; cellular defense response; immune response; positive regulation of alpha-beta T cell proliferation; blood vessel remodeling; positive regulation of T cell activation; negative regulation of eosinophil degranulation; positive regulation of inflammatory response