IL-10 elispot kit
Human IL-10 ELISpot Kit
Reactivity
Human. No cross reactivity with other human cytokines. Cross reactivity with simian IL-10. No cross reactivity with viral IL-10.
Synonyms
IL-10; Human IL-10 ELISpot Kit; IL-10 elispot kit
Reactivity
Human. No cross reactivity with other human cytokines. Cross reactivity with simian IL-10. No cross reactivity with viral IL-10.
Specificity
The assay recognizes natural human IL-10. To define specificity of this ELISpot, several proteins were tested for cross reactivity. There was no cross reactivity observed for these proteins tested IL-1beta, IL-12, IFNgamma, IL-4, IL-6, TNFalpha, IL-8, IL-2 and IL-13. This testing was performed using the equivalent IL-10 antibody pair in an ELISA assay.
Sequence Length
168
Intended Uses
ELISpot is a highly specific immunoassay for the analysis of cytokine and other soluble molecule production and secretion from T-cells at a single cell level in conditions closely comparable to the in-vivo environment with minimal cell manipulation. This technique is designed to determine the frequency of cytokine producing cells under a given stimulation and the comparison of such frequency against a specific treatment or pathological state. The ELISpot assay constitutes an ideal tool in the investigation of Th1 / Th2 responses, vaccine development, viral infection monitoring and treatment, cancerology, infectious disease, autoimmune diseases and tranplantation. Utilising sandwich immuno-enzyme technology, LISpot assays can detect both secreted cytokines and single cells that simultaneously produce multiple cytokines. Cell secreted cytokines or soluble molecules are captured by coated antibodies avoiding diffusion in supernatant, protease degradation or binding on soluble membrane receptors. After cell removal, the captured cytokines are revealed by tracer antibodies and appropriate conjugates.
Related Product Information for IL-10 elispot kit
Principle of the assay: Capture antibodies highly specific for the analyte of interest are coated to the wells of a PVDF bottomed 96 well microtitre plate either during kit manufacture or in the laboratory. The plate is then blocked to minimise any non-antibody dependent unspecific binding and finally washed before adding the cells to be investigated. Cell suspension and stimulant are added to the coated and blocked microtitre plate and the plate incubated allowing the specific antibodies to bind any analytes produced. Biotinylated detection antibodies are then added which bind to the previously captured analyte. Enzyme conjugated streptavidin is added binding to the detection antibodies. Any excess unbound analyte and antibodies are removed by careful washing. Colour substrate is then applied to the wells resulting in coloured spots which can be quantified using appropriate analysis software or manually using microscopes.
Product Categories/Family for IL-10 elispot kit
References
Bain C. et al., J. Virol., 2004; 78(19): 10460 - 10469
Hudak S. et al., J. Immuno., 2002; 169(3): 1189 - 1196
Hudak S. et al., J. Immuno., 2002; 169(3): 1189 - 1196
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
IL-10
NCBI Official Symbol
SPPV_03
NCBI Protein Information
IL-10
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Product Notes
The Human. No cross reactivity with other human cytokines. Cross reactivity with simian IL-10. No cross reactivity with viral IL-10. IL-10 (Catalog #AAA335692) is an ELISpot Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA335692 ELISpot Kit recognizes Human. No cross reactivity with other human cytokines. Cross reactivity with simian IL-10. No cross reactivity with viral IL-10. IL-10. It is sometimes possible for the material contained within the vial of "IL-10, ELISpot Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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