IL-2 elispot set

Human IL-2 ELISpot Set

Cat. Num. AAA335732

• Gene Names: IL2; IL-2; TCGF; lymphokine
• Reactivity: Human. No cross reactivity with other human cytokines. Cross reactivity with simian IL-2.
• Synonyms: IL-2; Human IL-2 ELISpot Set; IL-2 (with sterile plate); IL-2 elispot set

• Reactivity: Human. No cross reactivity with other human cytokines. Cross reactivity with simian IL-2.
• Specificity: The assay recognizes natural human IL-2. To define specificity of this IL-2 antibody pair, several proteins were tested for cross reactivity. There was no cross reactivity observed for any protein tested (IL-1alpha, IL-1beta, IL-10 IL-12, IFNgamma, IL-4, IL-6, TNFalpha IL-8 and IL-13). This testing was performed using the equivalent human IL-2 antibody pair in an ELISA assay.
• Sequence Length: 41

• Intended Uses: ELISpot is a highly specific immunoassay for the analysis of cytokine and other soluble molecule production and secretion from T-cells at a single cell level in conditions closely comparable to the in-vivo environment with minimal cell manipulation. This technique is designed to determine the frequency of cytokine producing cells under a given stimulation and the comparison of such frequency against a specific treatment or pathological state. The ELISpot assay constitutes an ideal tool in the investigation of Th1 / Th2 responses, vaccine development, viral infection monitoring and treatment, cancerology, infectious disease, autoimmune diseases and tranplantation. Utilising sandwich immuno-enzyme technology, ELISpot assays can detect both secreted cytokines and single cells that simultaneously produce multiple cytokines. Cell secreted cytokines or soluble molecules are captured by coated antibodies avoiding diffusion in supernatant, protease degradation or binding on soluble membrane receptors. After cell removal, the captured cytokines are revealed by tracer antibodies and appropriate conjugates.

Related Product Information for IL-2 elispot set

Principle of the assay: A capture antibody highly specific for the analyte of interest is coated to the wells of a PVDF bottomed 96 well microtitre plate either during kit manufacture or in the laboratory. The plate is then blocked to minimise any non-antibody dependent unspecific binding and washed. Cell suspension and stimulant are added and the plate incubated allowing the specific antibodies to bind any analytes produced. Cells are then removed by washing prior to the addition of Biotinylated detection antibodies which bind to the previously captured analyte. Enzyme conjugated streptavidin is then added binding to the detection antibodies. Following incubation and washing substrate is then applied to the wells resulting in coloured spots which can be quantified using appropriate analysis software or manually using a microscope.

Product Categories/Family for IL-2 elispot set

Human ELISpot Set

References

Dosset, M. et al., Clin Cancer Res., 2012; 18(22): 6284-95
Rinaldi, M. et al.,Thorax, 2012; 10.1136/thoraxjnl-2011-200690
Van Gulck, E. R. et al, J. Virol,2008; 82(7): 3561-3573.

NCBI and Uniprot Product Information

• NCBI GI #: 4029234
• NCBI GeneID: 3558
• UniProt Accession #: O95974; P60568
• Molecular Weight: 4,762 Da
• NCBI Official Full Name: IL-2, partial
• NCBI Official Synonym Full Names: interleukin 2
• NCBI Official Symbol: IL2
• NCBI Official Synonym Symbols: IL-2; TCGF; lymphokine
• NCBI Protein Information: interleukin-2
• UniProt Protein Name: IL-2 protein
• UniProt Gene Name: IL-2
• UniProt Entry Name: O95974_HUMAN

NCBI Description

The protein encoded by this gene is a secreted cytokine that is important for the proliferation of T and B lymphocytes. The receptor of this cytokine is a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL4) and interleukin 7 (IL7). The expression of this gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a similar gene in mice leads to ulcerative colitis-like disease, which suggests an essential role of this gene in the immune response to antigenic stimuli. [provided by RefSeq, Jul 2008]

Uniprot Description

IL2: Produced by T-cells in response to antigenic or mitogenic stimulation, this protein is required for T-cell proliferation and other activities crucial to regulation of the immune response. Can stimulate B-cells, monocytes, lymphokine- activated killer cells, natural killer cells, and glioma cells. A chromosomal aberration involving IL2 is found in a form of T-cell acute lymphoblastic leukemia (T-ALL). Translocation t(4;16)(q26;p13) with involves TNFRSF17. Belongs to the IL-2 family.

Protein type: Secreted, signal peptide; Secreted; Cytokine; Oncoprotein

Chromosomal Location of Human Ortholog: 4q26-q27

Cellular Component: extracellular space; extracellular region

Molecular Function: growth factor activity; interleukin-2 receptor binding; cytokine activity; kinase activator activity; carbohydrate binding; glycosphingolipid binding; kappa-type opioid receptor binding

Biological Process: positive regulation of isotype switching to IgG isotypes; negative regulation of heart contraction; natural killer cell activation; positive regulation of activated T cell proliferation; negative regulation of lymphocyte proliferation; elevation of cytosolic calcium ion concentration; cell-cell signaling; negative regulation of protein amino acid phosphorylation; positive regulation of cell proliferation; protein kinase C activation; positive regulation of B cell proliferation; cell adhesion; T cell differentiation; positive regulation of interleukin-17 production; regulation of T cell homeostatic proliferation; positive regulation of regulatory T cell differentiation; positive regulation of immunoglobulin secretion; positive regulation of cell growth; positive regulation of interferon-gamma production; positive regulation of tissue remodeling; positive regulation of tyrosine phosphorylation of Stat5 protein; negative regulation of inflammatory response; immune response; positive regulation of transcription from RNA polymerase II promoter; negative regulation of B cell apoptosis; negative regulation of apoptosis; positive regulation of inflammatory response