Human Vitamin D Receptor (VDR) RTU ELISA Kit | VDR rtu elisa kit

Human Vitamin D Receptor (VDR) ELISA Kit

Cat. Num. AAA4503279

• Gene Names: VDR; NR1I1; PPP1R163
• Reactivity: Human
• Synonyms: Vitamin D Receptor (VDR); Human Vitamin D Receptor (VDR) ELISA Kit; NR1I1; Nuclear Receptor Subfamily 1 Group I Member 1; Calcitriol Receptor; 1; 25-dihydroxyvitamin D3 receptor; VDR rtu elisa kit

• Reactivity: Human
• Sequence Length: 427

• Assay Type: Sandwich
• Samples: Tissue Homogenates, Cell Lysates or Other Biological Fluids.
• Detection Range: 0.625-40ng/mL
• Sensitivity: 0.224ng/mL
• RTU ELISA Kit's Advantage: The Ready-To-Use (RTU) Kits require fewer steps in setup and take less time to run. Detection Solutions are pre-diluted and total incubation time is shorter than many other ELISA Kits.
• Preparation and Storage: For unopened kits: All the reagents should be kept at 4 degree C upon receipt.; For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for VDR rtu elisa kit

Test Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to VDR. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to VDR. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain VDR, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of VDR in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 4507883
• NCBI GeneID: 7421
• NCBI Accession #: NP_000367.1
• NCBI GenBank Nucleotide #: NM_000376.2
• UniProt Accession #: P11473; Q5PSV3; B2R5Q1; G3V1V9
• Molecular Weight: 53,883 Da
• NCBI Official Full Name: vitamin D3 receptor isoform VDRA
• NCBI Official Synonym Full Names: vitamin D receptor
• NCBI Official Symbol: VDR
• NCBI Official Synonym Symbols: NR1I1; PPP1R163
• NCBI Protein Information: vitamin D3 receptor
• UniProt Protein Name: Vitamin D3 receptor
• Protein Family: Vitamin D3 receptor
• UniProt Gene Name: VDR
• UniProt Synonym Gene Names: NR1I1; VDR

NCBI Description

This gene encodes the nuclear hormone receptor for vitamin D3. This receptor also functions as a receptor for the secondary bile acid lithocholic acid. The receptor belongs to the family of trans-acting transcriptional regulatory factors and shows sequence similarity to the steroid and thyroid hormone receptors. Downstream targets of this nuclear hormone receptor are principally involved in mineral metabolism though the receptor regulates a variety of other metabolic pathways, such as those involved in the immune response and cancer. Mutations in this gene are associated with type II vitamin D-resistant rickets. A single nucleotide polymorphism in the initiation codon results in an alternate translation start site three codons downstream. Alternative splicing results in multiple transcript variants encoding different proteins. [provided by RefSeq, Feb 2011]

Uniprot Description

Nuclear receptor for calcitriol, the active form of vitamin D3 which mediates the action of this vitamin on cells. Enters the nucleus upon vitamin D3 binding where it forms heterodimers with the retinoid X receptor/RXR. The VDR-RXR heterodimers bind to specific response elements on DNA and activate the transcription of vitamin D3-responsive target genes. Recruited to promoters via its interaction with BAZ1B/WSTF which mediates the interaction with acetylated histones, an essential step for VDR-promoter association. Plays a central role in calcium homeostasis.