Human Ubiquitin A 52 Residue Ribosomal Protein Fusion Product 1 (UBA52) RTU ELISA Kit | UBA52 rtu elisa kit

Human Ubiquitin A 52 Residue Ribosomal Protein Fusion Product 1 (UBA52) ELISA Kit

Cat. Num. AAA4503172

• Gene Names: UBA52; L40; CEP52; RPL40; HUBCEP52
• Reactivity: Human
• Synonyms: Ubiquitin A 52 Residue Ribosomal Protein Fusion Product 1 (UBA52); Human Ubiquitin A 52 Residue Ribosomal Protein Fusion Product 1 (UBA52) ELISA Kit; CEP52; HUBCEP52; RPL40; 60S Ribosomal Protein L40; Ubiquitin Carboxyl Extension Protein 52; UBA52 rtu elisa kit

• Reactivity: Human
• Sequence Length: 128

• Assay Type: Sandwich
• Samples: Tissue Homogenates or Other Biological Fluids.
• Detection Range: 0.312-20ng/mL
• Sensitivity: 0.116ng/mL
• RTU ELISA Kit's Advantage: The Ready-To-Use (RTU) Kits require fewer steps in setup and take less time to run. Detection Solutions are pre-diluted and total incubation time is shorter than many other ELISA Kits.
• Preparation and Storage: For unopened kits: All the reagents should be kept at 4 degree C upon receipt.; For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for UBA52 rtu elisa kit

Test Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to UBA52. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to UBA52. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain UBA52, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of UBA52 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 77539055
• NCBI GeneID: 7311
• NCBI Accession #: NP_001029102.1
• NCBI GenBank Nucleotide #: NM_001033930.2
• UniProt Accession #: P62987; P02248; P02249; P02250; P14793; P62988; Q29120; Q6LBL4; Q6LDU5; Q8WYN8; Q91887; Q91888
• Molecular Weight: 14,728 Da
• NCBI Official Full Name: ubiquitin-60S ribosomal protein L40 isoform 1
• NCBI Official Synonym Full Names: ubiquitin A-52 residue ribosomal protein fusion product 1
• NCBI Official Symbol: UBA52
• NCBI Official Synonym Symbols: L40; CEP52; RPL40; HUBCEP52
• NCBI Protein Information: ubiquitin-60S ribosomal protein L40
• UniProt Protein Name: Ubiquitin-60S ribosomal protein L40
• UniProt Gene Name: UBA52
• UniProt Synonym Gene Names: UBCEP2

NCBI Description

Ubiquitin is a highly conserved nuclear and cytoplasmic protein that has a major role in targeting cellular proteins for degradation by the 26S proteosome. It is also involved in the maintenance of chromatin structure, the regulation of gene expression, and the stress response. Ubiquitin is synthesized as a precursor protein consisting of either polyubiquitin chains or a single ubiquitin moiety fused to an unrelated protein. This gene encodes a fusion protein consisting of ubiquitin at the N terminus and ribosomal protein L40 at the C terminus, a C-terminal extension protein (CEP). Multiple processed pseudogenes derived from this gene are present in the genome. [provided by RefSeq, Jul 2008]

Uniprot Description

Ubiquitin: Exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked: Lys-6-linked may be involved in DNA repair; Lys-11-linked is involved in ERAD (endoplasmic reticulum-associated degradation) and in cell-cycle regulation; Lys-29-linked is involved in lysosomal degradation; Lys-33-linked is involved in kinase modification; Lys-48-linked is involved in protein degradation via the proteasome; Lys-63-linked is involved in endocytosis, DNA-damage responses as well as in signaling processes leading to activation of the transcription factor NF-kappa-B. Linear polymer chains formed via attachment by the initiator Met lead to cell signaling. Ubiquitin is usually conjugated to Lys residues of target proteins, however, in rare cases, conjugation to Cys or Ser residues has been observed. When polyubiquitin is free (unanchored-polyubiquitin), it also has distinct roles, such as in activation of protein kinases, and in signaling.