Human Transportin 1 (TNPO1) RTU ELISA Kit | TNPO1 rtu elisa kit

Human Transportin 1 (TNPO1) ELISA Kit

Cat. Num. AAA4503030

• Gene Names: TNPO1; MIP; TRN; IPO2; MIP1; KPNB2
• Reactivity: Human
• Synonyms: Transportin 1 (TNPO1); Human Transportin 1 (TNPO1) ELISA Kit; MIP1; IPO2; KPNB2; MIP; TRN; M9 region interaction protein 1; Importin 2; Karyopherin Beta 2; TNPO1 rtu elisa kit

• Reactivity: Human
• Sequence Length: 898

• Assay Type: Sandwich
• Samples: Serum, Plasma, Tissue Homogenates or Other Biological Fluids.
• Detection Range: 3.12-200ng/mL
• Sensitivity: 1.06ng/mL
• RTU ELISA Kit's Advantage: The Ready-To-Use (RTU) Kits require fewer steps in setup and take less time to run. Detection Solutions are pre-diluted and total incubation time is shorter than many other ELISA Kits.
• Preparation and Storage: For unopened kits: All the reagents should be kept at 4 degree C upon receipt.; For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for TNPO1 rtu elisa kit

Test Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to TNPO1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to TNPO1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain TNPO1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of TNPO1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 133925811
• NCBI GeneID: 3842
• NCBI Accession #: NP_002261.3
• NCBI GenBank Nucleotide #: NM_002270.3
• UniProt Accession #: Q92973; Q92957; Q92975; B4DVC6
• Molecular Weight: 96,901 Da
• NCBI Official Full Name: transportin-1 isoform 1
• NCBI Official Synonym Full Names: transportin 1
• NCBI Official Symbol: TNPO1
• NCBI Official Synonym Symbols: MIP; TRN; IPO2; MIP1; KPNB2
• NCBI Protein Information: transportin-1
• UniProt Protein Name: Transportin-1
• UniProt Gene Name: TNPO1
• UniProt Synonym Gene Names: KPNB2; MIP1; TRN; MIP

NCBI Description

This gene encodes the beta subunit of the karyopherin receptor complex which interacts with nuclear localization signals to target nuclear proteins to the nucleus. The karyopherin receptor complex is a heterodimer of an alpha subunit which recognizes the nuclear localization signal and a beta subunit which docks the complex at nucleoporins. Alternate splicing of this gene results in two transcript variants encoding different proteins. [provided by RefSeq, Jul 2008]

Uniprot Description

Functions in nuclear protein import as nuclear transport receptor. Serves as receptor for nuclear localization signals (NLS) in cargo substrates (PubMed:24753571). Is thought to mediate docking of the importin/substrate complex to the nuclear pore complex (NPC) through binding to nucleoporin and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to the importin, the importin/substrate complex dissociates and importin is re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus (). Involved in nuclear import of M9-containing proteins. In vitro, binds directly to the M9 region of the heterogeneous nuclear ribonucleoproteins (hnRNP), A1 and A2 and mediates their nuclear import. Appears also to be involved in hnRNP A1/A2 nuclear export. Mediates the nuclear import of ribosomal proteins RPL23A, RPS7 and RPL5. Binds to a beta-like import receptor binding (BIB) domain of RPL23A. In vitro, mediates nuclear import of H2A, H2B, H3 and H4 histones, and SRP19 (). Mediates nuclear import of ADAR/ADAR1 isoform 1 and isoform 5 in a RanGTP-dependent manner (PubMed:19124606, PubMed:24753571).