Human Neutrophil Activating Protein 2 (NAP2) RTU ELISA Kit | NAP2 rtu elisa kit

Human Neutrophil Activating Protein 2 (NAP2) ELISA Kit

Cat. Num. AAA4503881

• Gene Names: NAP1L4; NAP2; NAP2L; hNAP2; NAP1L4b
• Reactivity: Human
• Synonyms: Neutrophil Activating Protein 2 (NAP2); Human Neutrophil Activating Protein 2 (NAP2) ELISA Kit; Nucleosome assembly protein 2; NAP-2; NAP2 rtu elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of NAP2. No significant cross-reactivity or interference between NAP2 and analogues was observed.
• Sequence Length: 375

• Samples: Human Serum, Plasma, Tissue Homogenates Or Other Biological Fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 31.2-2000pg/mL
• Sensitivity: 10.1pg/mL.
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level NAP2 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level NAP2 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for NAP2 rtu elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of NAP2 in human serum, plasma, tissue homogenates or other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to NAP2. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to NAP2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain NAP2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of NAP2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 2498672
• NCBI GeneID: 4676
• UniProt Accession #: Q99733
• NCBI Official Full Name: Nucleosome assembly protein 1-like 4
• NCBI Official Synonym Full Names: nucleosome assembly protein 1 like 4
• NCBI Official Symbol: NAP1L4
• NCBI Official Synonym Symbols: NAP2; NAP2L; hNAP2; NAP1L4b
• NCBI Protein Information: nucleosome assembly protein 1-like 4
• UniProt Protein Name: Nucleosome assembly protein 1-like 4
• UniProt Gene Name: NAP1L4
• UniProt Synonym Gene Names: NAP2; NAP-2
• UniProt Entry Name: NP1L4_HUMAN

NCBI Description

This gene encodes a member of the nucleosome assembly protein (NAP) family which can interact with both core and linker histones. It can shuttle between the cytoplasm and nucleus, suggesting a role as a histone chaperone. This gene is one of several located near the imprinted gene domain of 11p15.5, an important tumor-suppressor gene region. Alterations in this region have been associated with the Beckwith-Wiedemann syndrome, Wilms tumor, rhabdomyosarcoma, adrenocortical carcinoma, and lung, ovarian, and breast cancer. [provided by RefSeq, Jul 2008]

Uniprot Description

NAP1L4: a member of the nucleosome assembly protein (NAP) family which can interact with both core and linker histones. It can shuttle between the cytoplasm and nucleus, suggesting a role as a histone chaperone. This gene is one of several located near the imprinted gene domain of 11p15.5, an important tumor-suppressor gene region. Alterations in this region have been associated with the Beckwith-Wiedemann syndrome, Wilms tumor, rhabdomyosarcoma, adrenocortical carcinoma, and lung, ovarian, and breast cancer. [provided by RefSeq, Jul 2008]

Protein type: Chaperone

Chromosomal Location of Human Ortholog: 11p15.5

Cellular Component: cytoplasm; nucleus

Molecular Function: protein binding; unfolded protein binding

Biological Process: nucleosome assembly