Human Microphthalmia Associated Transcription Factor (MITF) RTU ELISA Kit | MITF rtu elisa kit

Human Microphthalmia Associated Transcription Factor (MITF) ELISA Kit

Cat. Num. AAA4501046

• Gene Names: MITF; MI; WS2; CMM8; WS2A; COMMAD; bHLHe32
• Reactivity: Human
• Synonyms: Microphthalmia Associated Transcription Factor (MITF); Human Microphthalmia Associated Transcription Factor (MITF) ELISA Kit; WS2A; MI; bHLHe32; Homolog Of Mouse Microphthalmia; Class E basic helix-loop-helix protein 32; MITF rtu elisa kit

• Reactivity: Human
• Sequence Length: 419

• Assay Type: Sandwich
• Samples: Tissue Homogenates, Cell Lysates or Other Biological Fluids.
• Detection Range: 0.156-10ng/mL
• Sensitivity: 0.059ng/mL
• RTU ELISA Kit's Advantage: The Ready-To-Use (RTU) Kits require fewer steps in setup and take less time to run. Detection Solutions are pre-diluted and total incubation time is shorter than many other ELISA Kits.
• Preparation and Storage: For unopened kits: All the reagents should be kept at 4 degree C upon receipt.; For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for MITF rtu elisa kit

Test Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to MITF. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to MITF. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain MITF, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of MITF in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 4557755
• NCBI GeneID: 4286
• NCBI Accession #: NP_000239.1
• NCBI GenBank Nucleotide #: NM_000248.3
• UniProt Accession #: O75030; Q14841; Q9P2V0; Q9P2V1; Q9P2V2; Q9P2Y8; B4DJL2; D3K197; E9PFN0
• Molecular Weight: 52,527 Da
• NCBI Official Full Name: microphthalmia-associated transcription factor isoform 4
• NCBI Official Synonym Full Names: melanogenesis associated transcription factor
• NCBI Official Symbol: MITF
• NCBI Official Synonym Symbols: MI; WS2; CMM8; WS2A; COMMAD; bHLHe32
• NCBI Protein Information: microphthalmia-associated transcription factor
• UniProt Protein Name: Microphthalmia-associated transcription factor
• Protein Family: Microphthalmia-associated transcription factor
• UniProt Gene Name: MITF
• UniProt Synonym Gene Names: BHLHE32; bHLHe32

NCBI Description

The protein encoded by this gene is a transcription factor that contains both basic helix-loop-helix and leucine zipper structural features. The encoded protein regulates melanocyte development and is responsible for pigment cell-specific transcription of the melanogenesis enzyme genes. Heterozygous mutations in the this gene cause auditory-pigmentary syndromes, such as Waardenburg syndrome type 2 and Tietz syndrome. [provided by RefSeq, Aug 2017]

Uniprot Description

Transcription factor that regulates the expression of genes with essential roles in cell differentiation, proliferation and survival. Binds to M-boxes (5'-TCATGTG-3') and symmetrical DNA sequences (E-boxes) (5'-CACGTG-3') found in the promoters of target genes, such as BCL2 and tyrosinase (TYR). Plays an important role in melanocyte development by regulating the expression of tyrosinase (TYR) and tyrosinase-related protein 1 (TYRP1). Plays a critical role in the differentiation of various cell types, such as neural crest-derived melanocytes, mast cells, osteoclasts and optic cup-derived retinal pigment epithelium.