Human Metallothionein 1 (MT1) RTU ELISA Kit | MT1 rtu elisa kit

Human Metallothionein 1 (MT1) ELISA Kit

Cat. Num. AAA4501173

• Gene Names: MT1A; MT1; MTC; MT1S; MT-1A; MT-IA
• Reactivity: Human
• Synonyms: Metallothionein 1 (MT1); Human Metallothionein 1 (MT1) ELISA Kit; MT1A; MT1S; MTC; MT1 rtu elisa kit

• Reactivity: Human
• Sequence Length: 61

• Assay Type: Sandwich
• Samples: Serum, Plasma, Tissue Homogenates or Other Biological Fluids.
• Detection Range: 15.6-1000pg/mL
• Sensitivity: 6.2pg/mL
• RTU ELISA Kit's Advantage: The Ready-To-Use (RTU) Kits require fewer steps in setup and take less time to run. Detection Solutions are pre-diluted and total incubation time is shorter than many other ELISA Kits.
• Preparation and Storage: For unopened kits: All the reagents should be kept at 4 degree C upon receipt.; For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for MT1 rtu elisa kit

Test Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to MT1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to MT1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain MT1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of MT1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 71274113
• NCBI GeneID: 4489
• NCBI Accession #: NP_005937.2
• NCBI GenBank Nucleotide #: NM_005946.2
• UniProt Accession #: P04731; Q86YX5
• Molecular Weight: 6,120 Da
• NCBI Official Full Name: metallothionein-1A
• NCBI Official Synonym Full Names: metallothionein 1A
• NCBI Official Symbol: MT1A
• NCBI Official Synonym Symbols: MT1; MTC; MT1S; MT-1A; MT-IA
• NCBI Protein Information: metallothionein-1A
• UniProt Protein Name: Metallothionein-1A
• Protein Family: Sphingolipid C9-methyltransferase
• UniProt Gene Name: MT1A
• UniProt Synonym Gene Names: MT1S; MT-1A; MT-IA

NCBI Description

This gene is a member of the metallothionein family of genes. Proteins encoded by this gene family are low in molecular weight, are cysteine-rich, lack aromatic residues, and bind divalent heavy metal ions. The conserved cysteine residues co-ordinate metal ions using mercaptide linkages. These proteins act as anti-oxidants, protect against hydroxyl free radicals, are important in homeostatic control of metal in the cell, and play a role in detoxification of heavy metals. Disruption of two metallothionein genes in mouse resulted in defects in protection against heavy metals, oxidative stress, immune reactions, carcinogens, and displayed obesity. [provided by RefSeq, Sep 2017]

Uniprot Description

Metallothioneins have a high content of cysteine residues that bind various heavy metals; these proteins are transcriptionally regulated by both heavy metals and glucocorticoids.