Human Matrix Metalloproteinase 16 (MMP16) RTU ELISA Kit | MMP16 rtu elisa kit

Human Matrix Metalloproteinase 16 (MMP16) ELISA Kit

Cat. Num. AAA4501076

• Gene Names: MMP16; MMP-X2; C8orf57; MT-MMP2; MT-MMP3; MT3-MMP
• Reactivity: Human
• Synonyms: Matrix Metalloproteinase 16 (MMP16); Human Matrix Metalloproteinase 16 (MMP16) ELISA Kit; MT3-MMP; MMP16; MMP-X2; MT-MMP2; MT-MMP3; Membrane-type matrix metalloproteinase 3; Membrane-type-3 matrix metalloproteinase; MMP16 rtu elisa kit

• Reactivity: Human
• Sequence Length: 607

• Assay Type: Sandwich
• Samples: Serum, Plasma, Tissue Homogenates or Other Biological Fluids.
• Detection Range: 0.156-10ng/mL
• Sensitivity: 0.063ng/mL
• RTU ELISA Kit's Advantage: The Ready-To-Use (RTU) Kits require fewer steps in setup and take less time to run. Detection Solutions are pre-diluted and total incubation time is shorter than many other ELISA Kits.
• Preparation and Storage: For unopened kits: All the reagents should be kept at 4 degree C upon receipt.; For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for MMP16 rtu elisa kit

Test Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to MMP16. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to MMP16. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain MMP16, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of MMP16 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 13027802
• NCBI GeneID: 4325
• NCBI Accession #: NP_005932.2
• NCBI GenBank Nucleotide #: NM_005941.4
• UniProt Accession #: P51512; Q14824; Q52H48; B2RAN7
• Molecular Weight: 52,515 Da
• NCBI Official Full Name: matrix metalloproteinase-16
• NCBI Official Synonym Full Names: matrix metallopeptidase 16
• NCBI Official Symbol: MMP16
• NCBI Official Synonym Symbols: MMP-X2; C8orf57; MT-MMP2; MT-MMP3; MT3-MMP
• NCBI Protein Information: matrix metalloproteinase-16
• UniProt Protein Name: Matrix metalloproteinase-16
• Protein Family: Matrix metalloproteinase
• UniProt Gene Name: MMP16
• UniProt Synonym Gene Names: MMP-16; MT-MMP 3; MTMMP3; MT3-MMP; MT3MMP

NCBI Description

Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The encoded protein activates MMP2 by cleavage. This gene was once referred to as MT-MMP2, but was renamed as MT-MMP3 or MMP16. [provided by RefSeq, Oct 2010]

Uniprot Description

Endopeptidase that degrades various components of the extracellular matrix, such as collagen type III and fibronectin. Activates progelatinase A. Involved in the matrix remodeling of blood vessels. Isoform short cleaves fibronectin and also collagen type III, but at lower rate. It has no effect on type I, II, IV and V collagen. However, upon interaction with CSPG4, it may be involved in degradation and invasion of type I collagen by melanoma cells.