Human Lipoprotein, a (Lpa) RTU ELISA Kit | Lpa rtu elisa kit

Human Lipoprotein, a (Lpa) ELISA Kit

Cat. Num. AAA4500817

• Gene Names: LPA; LP; AK38; APOA
• Reactivity: Human
• Synonyms: Lipoprotein; a (Lpa); Human Lipoprotein; a (Lpa) ELISA Kit; Lp(a); AK38; APOA; LP; Sinking Pre-beta-Lipoprotein; Lpa rtu elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Lpa. No significant cross-reactivity or interference between Lpa and analogues was observed.
• Sequence Length: 4548

• Samples: Serum, plasma, tissue homogenates or other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 0.234-15ng/mL
• Sensitivity: < 0.09ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Lpa were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Lpa were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kits: All the reagents should be kept at 4 degree C upon receipt.; For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.

Typical Testing Data/Standard Curve (for reference only)

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Related Product Information for Lpa rtu elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of Lpa in human serum, plasma, tissue homogenates or other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to Lpa. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to Lpa. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Lpa, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Lpa in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 114062
• NCBI GeneID: 4018
• UniProt Accession #: P08519; Q5VTD7; Q9UD88
• Molecular Weight: 501,319 Da
• NCBI Official Full Name: Apolipoprotein(a)
• NCBI Official Synonym Full Names: lipoprotein(a)
• NCBI Official Symbol: LPA
• NCBI Official Synonym Symbols: LP; AK38; APOA
• NCBI Protein Information: apolipoprotein(a)
• UniProt Protein Name: Apolipoprotein(a)
• Protein Family: Late promoter-activating protein
• UniProt Gene Name: LPA
• UniProt Synonym Gene Names: Apo(a); Lp(a)

NCBI Description

The protein encoded by this gene is a serine proteinase that inhibits the activity of tissue-type plasminogen activator I. The encoded protein constitutes a substantial portion of lipoprotein(a) and is proteolytically cleaved, resulting in fragments that attach to atherosclerotic lesions and promote thrombogenesis. Elevated plasma levels of this protein are linked to atherosclerosis. Depending on the individual, the encoded protein contains 2-43 copies of kringle-type domains. The allele represented here contains 15 copies of the kringle-type repeats and corresponds to that found in the reference genome sequence. [provided by RefSeq, Dec 2009]

Uniprot Description

Apo(a) is the main constituent of lipoprotein(a) (Lp(a)). It has serine proteinase activity and is able of autoproteolysis. Inhibits tissue-type plasminogen activator 1. Lp(a) may be a ligand for megalin/Gp 330.