Human High Affinity Choline Transporter (ChT) RTU ELISA Kit | ChT rtu elisa kit

Human High Affinity Choline Transporter (ChT) ELISA Kit

Cat. Num. AAA4503502

• Gene Names: SLC5A7; CHT; CHT1; CMS20; HMN7A
• Reactivity: Human
• Synonyms: High Affinity Choline Transporter (ChT); Human High Affinity Choline Transporter (ChT) ELISA Kit; SLC5A7; hCHT; CHT1; Solute Carrier Family 5 Member 7; Choline Transporter; Hemicholinium-3-sensitive choline transporter; ChT rtu elisa kit

• Reactivity: Human
• Sequence Length: 580

• Assay Type: Sandwich
• Samples: Tissue Homogenates, Cell Lysates or Other Biological Fluids.
• Detection Range: 0.156-10ng/mL
• Sensitivity: 0.057ng/mL
• RTU ELISA Kit's Advantage: The Ready-To-Use (RTU) Kits require fewer steps in setup and take less time to run. Detection Solutions are pre-diluted and total incubation time is shorter than many other ELISA Kits.
• Preparation and Storage: For unopened kits: All the reagents should be kept at 4 degree C upon receipt.; For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ChT rtu elisa kit

Test Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to ChT. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to ChT. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain ChT, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of ChT in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 756761781
• NCBI GeneID: 60482
• NCBI Accession #: NP_001291934.1
• NCBI GenBank Nucleotide #: NM_001305005.2
• UniProt Accession #: Q9GZV3; Q53TF2
• Molecular Weight: 63,204 Da
• NCBI Official Full Name: high affinity choline transporter 1 isoform a
• NCBI Official Synonym Full Names: solute carrier family 5 member 7
• NCBI Official Symbol: SLC5A7
• NCBI Official Synonym Symbols: CHT; CHT1; CMS20; HMN7A
• NCBI Protein Information: high affinity choline transporter 1
• UniProt Protein Name: High affinity choline transporter 1
• UniProt Gene Name: SLC5A7
• UniProt Synonym Gene Names: CHT1; CHT

NCBI Description

This gene encodes a sodium ion- and chloride ion-dependent high-affinity transporter that mediates choline uptake for acetylcholine synthesis in cholinergic neurons. The protein transports choline from the extracellular space into presynaptic terminals for synthesis into acetylcholine. Increased choline uptake results from increased density of this protein in synaptosomal plasma membranes in response to depolarization of cholinergic terminals. Dysfunction of cholinergic signaling has been implicated in various disorders including depression, attention-deficit disorder, and schizophrenia. An allelic variant of this gene is associated with autosomal dominant distal hereditary motor neuronopathy type VIIA. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2015]

Uniprot Description

Transmembrane transporter that imports choline from the extracellular space into the neuron with high affinity. Choline uptake is the rate-limiting step in acetylcholine synthesis. Sodium ion- and chloride ion-dependent.