Typical Testing Data/Standard Curve (for reference only)
Human Hepatocyte Nuclear Factor 1 Beta (HNF1b) RTU ELISA Kit | HNF1b rtu elisa kit
Human Hepatocyte Nuclear Factor 1 Beta (HNF1b) ELISA Kit
Gene Names
HNF1B; FJHN; HNF2; LFB3; TCF2; HPC11; LF-B3; MODY5; TCF-2; VHNF1; HNF-1B; HNF1beta; HNF-1-beta
Reactivity
Human
Synonyms
Hepatocyte Nuclear Factor 1 Beta (HNF1b); Human Hepatocyte Nuclear Factor 1 Beta (HNF1b) ELISA Kit; FJHN; TCF2; HNF1beta; HNF2; LFB3; LF-B3; MODY5; VHNF1; Transcription Factor 2; Hepatic; Variant Hepatic Nuclear Factor; Homeoprotein LFB3; HNF1b rtu elisa kit
Reactivity
Human
Sequence Length
557
Assay Type
Sandwich
Samples
Tissue Homogenates or Other Biological Fluids.
Detection Range
0.156-10ng/mL
Sensitivity
0.047ng/mL
RTU ELISA Kit's Advantage
The Ready-To-Use (RTU) Kits require fewer steps in setup and take less time to run. Detection Solutions are pre-diluted and total incubation time is shorter than many other ELISA Kits.
Preparation and Storage
For unopened kits: All the reagents should be kept at 4 degree C upon receipt.
For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.
For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for HNF1b rtu elisa kit
Test Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to HNF1b. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to HNF1b. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain HNF1b, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of HNF1b in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
46,907 Da
NCBI Official Full Name
hepatocyte nuclear factor 1-beta isoform 1
NCBI Official Synonym Full Names
HNF1 homeobox B
NCBI Official Symbol
HNF1B
NCBI Official Synonym Symbols
FJHN; HNF2; LFB3; TCF2; HPC11; LF-B3; MODY5; TCF-2; VHNF1; HNF-1B; HNF1beta; HNF-1-beta
NCBI Protein Information
hepatocyte nuclear factor 1-beta
UniProt Protein Name
Hepatocyte nuclear factor 1-beta
Protein Family
UniProt Gene Name
HNF1B
UniProt Synonym Gene Names
TCF2; HNF-1-beta; HNF-1B; TCF-2; vHNF1
NCBI Description
This gene encodes a member of the homeodomain-containing superfamily of transcription factors. The protein binds to DNA as either a homodimer, or a heterodimer with the related protein hepatocyte nuclear factor 1-alpha. The gene has been shown to function in nephron development, and regulates development of the embryonic pancreas. Mutations in this gene result in renal cysts and diabetes syndrome and noninsulin-dependent diabetes mellitus, and expression of this gene is altered in some types of cancer. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Sep 2009]
Uniprot Description
Transcription factor, probably binds to the inverted palindrome 5'-GTTAATNATTAAC-3'.
Research Articles on HNF1b
Similar Products
Product Notes
The Human HNF1b hnf1b (Catalog #AAA459884) is a RTU ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA459884 RTU ELISA Kit recognizes Human HNF1b. It is sometimes possible for the material contained within the vial of "Hepatocyte Nuclear Factor 1 Beta (HNF1b), RTU ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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