Human Ghrelin (GHRL) RTU ELISA Kit | GHRL rtu elisa kit

Human Ghrelin (GHRL) ELISA Kit

Cat. Num. AAA459577

• Gene Names: GHRL; MTLRP
• Reactivity: Human
• Synonyms: Ghrelin (GHRL); Human Ghrelin (GHRL) ELISA Kit; MTLRP; Growth Hormone-Releasing Peptide; GHRL rtu elisa kit

• Reactivity: Human
• Sequence Length: 116

• Assay Type: Competitive Inhibition
• Samples: Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates or Other Biological Fluids.
• Detection Range: 0.156-10ng/mL
• Sensitivity: 0.047ng/mL
• RTU ELISA Kit's Advantage: The Ready-To-Use (RTU) Kits require fewer steps in setup and take less time to run. Detection Solutions are pre-diluted and total incubation time is shorter than many other ELISA Kits.
• Preparation and Storage: For unopened kits: All the reagents should be kept at 4 degree C upon receipt.; For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.

Related Product Information for GHRL rtu elisa kit

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to GHRL has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled GHRL and unlabeled GHRL (Standards or samples) with the pre-coated antibody specific to GHRL. After incubation the unbound conjugate is washed off. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of GHRL in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of GHRL in the sample.

NCBI and Uniprot Product Information

• NCBI GI #: 201860279
• NCBI GeneID: 51738
• NCBI Accession #: NP_001128413.1
• NCBI GenBank Nucleotide #: NM_001134941.2
• UniProt Accession #: Q9UBU3; Q86YP8; Q8TAT9; Q9H3R3; A8CF34; A8CF38; A8CF42; A8DN29; A8DN30
• Molecular Weight: 9,972 Da
• NCBI Official Full Name: appetite-regulating hormone isoform 2 preproprotein
• NCBI Official Synonym Full Names: ghrelin and obestatin prepropeptide
• NCBI Official Symbol: GHRL
• NCBI Official Synonym Symbols: MTLRP
• NCBI Protein Information: appetite-regulating hormone
• UniProt Protein Name: Appetite-regulating hormone
• Protein Family: Ghrelin
• UniProt Gene Name: GHRL
• UniProt Synonym Gene Names: MTLRP; Ghrelin

NCBI Description

This gene encodes the ghrelin-obestatin preproprotein that is cleaved to yield two peptides, ghrelin and obestatin. Ghrelin is a powerful appetite stimulant and plays an important role in energy homeostasis. Its secretion is initiated when the stomach is empty, whereupon it binds to the growth hormone secretagogue receptor in the hypothalamus which results in the secretion of growth hormone (somatotropin). Ghrelin is thought to regulate multiple activities, including hunger, reward perception via the mesolimbic pathway, gastric acid secretion, gastrointestinal motility, and pancreatic glucose-stimulated insulin secretion. It was initially proposed that obestatin plays an opposing role to ghrelin by promoting satiety and thus decreasing food intake, but this action is still debated. Recent reports suggest multiple metabolic roles for obestatin, including regulating adipocyte function and glucose metabolism. Alternative splicing results in multiple transcript variants. In addition, antisense transcripts for this gene have been identified and may potentially regulate ghrelin-obestatin preproprotein expression. [provided by RefSeq, Nov 2014]

Uniprot Description

Ghrelin is the ligand for growth hormone secretagogue receptor type 1 (GHSR). Induces the release of growth hormone from the pituitary. Has an appetite-stimulating effect, induces adiposity and stimulates gastric acid secretion. Involved in growth regulation.Obestatin may be the ligand for GPR39. May have an appetite-reducing effect resulting in decreased food intake. May reduce gastric emptying activity and jejunal motility ().