Human Frizzled Homolog 6 (FZD6) RTU ELISA Kit | FZD6 rtu elisa kit

Human Frizzled Homolog 6 (FZD6) ELISA Kit

Cat. Num. AAA459431

• Gene Names: FZD6; FZ6; FZ-6; HFZ6; NDNC10
• Reactivity: Human
• Synonyms: Frizzled Homolog 6 (FZD6); Human Frizzled Homolog 6 (FZD6) ELISA Kit; Hfz6; FZD6 rtu elisa kit

• Reactivity: Human
• Sequence Length: 706

• Assay Type: Sandwich
• Samples: Tissue Homogenates or Other Biological Fluids.
• Detection Range: 0.312-20ng/mL
• Sensitivity: 0.119ng/mL
• RTU ELISA Kit's Advantage: The Ready-To-Use (RTU) Kits require fewer steps in setup and take less time to run. Detection Solutions are pre-diluted and total incubation time is shorter than many other ELISA Kits.
• Preparation and Storage: For unopened kits: All the reagents should be kept at 4 degree C upon receipt.; For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for FZD6 rtu elisa kit

Test Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to FZD6. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to FZD6. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain FZD6, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of FZD6 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 257470999
• NCBI GeneID: 8323
• NCBI Accession #: NP_001158087.1
• NCBI GenBank Nucleotide #: NM_001164615.1
• UniProt Accession #: O60353; Q6N0A5; Q6P9C3; Q8WXR9; B4DRN0
• Molecular Weight: 75,579 Da
• NCBI Official Full Name: frizzled-6 isoform a
• NCBI Official Synonym Full Names: frizzled class receptor 6
• NCBI Official Symbol: FZD6
• NCBI Official Synonym Symbols: FZ6; FZ-6; HFZ6; NDNC10
• NCBI Protein Information: frizzled-6
• UniProt Protein Name: Frizzled-6
• Protein Family: Frizzled
• UniProt Gene Name: FZD6
• UniProt Synonym Gene Names: Fz-6; hFz6

NCBI Description

This gene represents a member of the 'frizzled' gene family, which encode 7-transmembrane domain proteins that are receptors for Wnt signaling proteins. The protein encoded by this family member contains a signal peptide, a cysteine-rich domain in the N-terminal extracellular region, and seven transmembrane domains, but unlike other family members, this protein does not contain a C-terminal PDZ domain-binding motif. This protein functions as a negative regulator of the canonical Wnt/beta-catenin signaling cascade, thereby inhibiting the processes that trigger oncogenic transformation, cell proliferation, and inhibition of apoptosis. Alternative splicing results in multiple transcript variants, some of which do not encode a protein with a predicted signal peptide.[provided by RefSeq, Aug 2011]

Uniprot Description

Receptor for Wnt proteins. Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues. Together with FZD3, is involved in the neural tube closure and plays a role in the regulation of the establishment of planar cell polarity (PCP), particularly in the orientation of asymmetric bundles of stereocilia on the apical faces of a subset of auditory and vestibular sensory cells located in the inner ear ().