Human DNA Methyltransferase 3B (DNMT3B) RTU ELISA Kit | DNMT3B rtu elisa kit

Human DNA Methyltransferase 3B (DNMT3B) ELISA Kit

Cat. Num. AAA458901

• Gene Names: DNMT3B; ICF; ICF1; M.HsaIIIB
• Reactivity: Human
• Synonyms: DNA Methyltransferase 3B (DNMT3B); Human DNA Methyltransferase 3B (DNMT3B) ELISA Kit; ICF; M.HsaIIIB; DNA methyltransferase HsaIIIB; DNMT3B rtu elisa kit

• Reactivity: Human
• Sequence Length: 728

• Assay Type: Sandwich
• Samples: Tissue Homogenates or Other Biological Fluids.
• Detection Range: 0.156-10ng/mL
• Sensitivity: 0.054ng/mL
• RTU ELISA Kit's Advantage: The Ready-To-Use (RTU) Kits require fewer steps in setup and take less time to run. Detection Solutions are pre-diluted and total incubation time is shorter than many other ELISA Kits.
• Preparation and Storage: For unopened kits: All the reagents should be kept at 4 degree C upon receipt.; For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for DNMT3B rtu elisa kit

Test Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to DNMT3B. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to DNMT3B. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain DNMT3B, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of DNMT3B in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 333440487
• NCBI GeneID: 1789
• NCBI Accession #: NP_001193984.1
• NCBI GenBank Nucleotide #: NM_001207055.1
• UniProt Accession #: Q9UBC3; Q9UBD4; Q9UJQ5; Q9UKA6; Q9UNE5; A2A2E2; B4DSM8; B4DSU1; E1P5M6; E1P5M7; E7EN63; E9PBF2
• Molecular Weight: 81,311 Da
• NCBI Official Full Name: DNA (cytosine-5)-methyltransferase 3B isoform 7
• NCBI Official Synonym Full Names: DNA methyltransferase 3 beta
• NCBI Official Symbol: DNMT3B
• NCBI Official Synonym Symbols: ICF; ICF1; M.HsaIIIB
• NCBI Protein Information: DNA (cytosine-5)-methyltransferase 3B
• UniProt Protein Name: DNA (cytosine-5)-methyltransferase 3B
• Protein Family: DNA
• UniProt Gene Name: DNMT3B
• UniProt Synonym Gene Names: Dnmt3b; DNA MTase HsaIIIB; M.HsaIIIB

NCBI Description

CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. This gene encodes a DNA methyltransferase which is thought to function in de novo methylation, rather than maintenance methylation. The protein localizes primarily to the nucleus and its expression is developmentally regulated. Mutations in this gene cause the immunodeficiency-centromeric instability-facial anomalies (ICF) syndrome. Eight alternatively spliced transcript variants have been described. The full length sequences of variants 4 and 5 have not been determined. [provided by RefSeq, May 2011]

Uniprot Description

Required for genome-wide de novo methylation and is essential for the establishment of DNA methylation patterns during development. DNA methylation is coordinated with methylation of histones. May preferentially methylates nucleosomal DNA within the nucleosome core region. May function as transcriptional co-repressor by associating with CBX4 and independently of DNA methylation. Seems to be involved in gene silencing (). In association with DNMT1 and via the recruitment of CTCFL/BORIS, involved in activation of BAG1 gene expression by modulating dimethylation of promoter histone H3 at H3K4 and H3K9. Isoforms 4 and 5 are probably not functional due to the deletion of two conserved methyltransferase motifs. Function as transcriptional corepressor by associating with ZHX1. Required for DUX4 silencing in somatic cells (PubMed:27153398).