Human Ataxia Telangiectasia Mutated (ATM) RTU ELISA Kit | ATM rtu elisa kit

Human Ataxia Telangiectasia Mutated (ATM) ELISA Kit

Cat. Num. AAA457808

• Gene Names: ATM; AT1; ATA; ATC; ATD; ATE; ATDC; TEL1; TELO1
• Reactivity: Human
• Synonyms: Ataxia Telangiectasia Mutated (ATM); Human Ataxia Telangiectasia Mutated (ATM) ELISA Kit; AT1; ATD; ATA; ATC; ATDC; ATE; TEL1; TELO1; Serine-protein kinase ATM; ATM rtu elisa kit

• Reactivity: Human
• Sequence Length: 3056

• Assay Type: Sandwich
• Samples: Serum, Plasma, Tissue Homogenates or Other Biological Fluids.
• Detection Range: 0.156-10ng/mL
• Sensitivity: 0.047ng/mL
• RTU ELISA Kit's Advantage: The Ready-To-Use (RTU) Kits require fewer steps in setup and take less time to run. Detection Solutions are pre-diluted and total incubation time is shorter than many other ELISA Kits.
• Preparation and Storage: For unopened kits: All the reagents should be kept at 4 degree C upon receipt.; For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ATM rtu elisa kit

Test Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to ATM. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to ATM. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain ATM, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of ATM in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 71902540
• NCBI GeneID: 472
• NCBI Accession #: NP_000042.3
• NCBI GenBank Nucleotide #: NM_000051.3
• UniProt Accession #: Q13315; O15429; Q12758; Q16551; Q93007; Q9NP02; Q9UCX7; B2RNX5
• Molecular Weight: 350,687 Da
• NCBI Official Full Name: serine-protein kinase ATM isoform a
• NCBI Official Synonym Full Names: ATM serine/threonine kinase
• NCBI Official Symbol: ATM
• NCBI Official Synonym Symbols: AT1; ATA; ATC; ATD; ATE; ATDC; TEL1; TELO1
• NCBI Protein Information: serine-protein kinase ATM
• UniProt Protein Name: Serine-protein kinase ATM
• Protein Family: Serine-protein kinase
• UniProt Gene Name: ATM
• UniProt Synonym Gene Names: A-T mutated

NCBI Description

The protein encoded by this gene belongs to the PI3/PI4-kinase family. This protein is an important cell cycle checkpoint kinase that phosphorylates; thus, it functions as a regulator of a wide variety of downstream proteins, including tumor suppressor proteins p53 and BRCA1, checkpoint kinase CHK2, checkpoint proteins RAD17 and RAD9, and DNA repair protein NBS1. This protein and the closely related kinase ATR are thought to be master controllers of cell cycle checkpoint signaling pathways that are required for cell response to DNA damage and for genome stability. Mutations in this gene are associated with ataxia telangiectasia, an autosomal recessive disorder. [provided by RefSeq, Aug 2010]

Uniprot Description

Serine/threonine protein kinase which activates checkpoint signaling upon double strand breaks (DSBs), apoptosis and genotoxic stresses such as ionizing ultraviolet A light (UVA), thereby acting as a DNA damage sensor. Recognizes the substrate consensus sequence [ST]-Q. Phosphorylates 'Ser-139' of histone variant H2AX/H2AFX at double strand breaks (DSBs), thereby regulating DNA damage response mechanism. Also plays a role in pre-B cell allelic exclusion, a process leading to expression of a single immunoglobulin heavy chain allele to enforce clonality and monospecific recognition by the B-cell antigen receptor (BCR) expressed on individual B-lymphocytes. After the introduction of DNA breaks by the RAG complex on one immunoglobulin allele, acts by mediating a repositioning of the second allele to pericentromeric heterochromatin, preventing accessibility to the RAG complex and recombination of the second allele. Also involved in signal transduction and cell cycle control. May function as a tumor suppressor. Necessary for activation of ABL1 and SAPK. Phosphorylates DYRK2, CHEK2, p53/TP53, FANCD2, NFKBIA, BRCA1, CTIP, nibrin (NBN), TERF1, RAD9 and DCLRE1C. May play a role in vesicle and/or protein transport. Could play a role in T-cell development, gonad and neurological function. Plays a role in replication-dependent histone mRNA degradation. Binds DNA ends. Phosphorylation of DYRK2 in nucleus in response to genotoxic stress prevents its MDM2-mediated ubiquitination and subsequent proteasome degradation. Phosphorylates ATF2 which stimulates its function in DNA damage response.