Typical Testing Data/Standard Curve (for reference only)
Human Alkaline Phosphatase, Placental (ALPP) RTU ELISA Kit | ALPP rtu elisa kit
Human Alkaline Phosphatase, Placental (ALPP) ELISA Kit
Gene Names
ALPP; ALP; PALP; PLAP; PLAP-1
Reactivity
Human
Synonyms
Alkaline Phosphatase; Placental (ALPP); Human Alkaline Phosphatase; Placental (ALPP) ELISA Kit; PLAP1; ALP; ALPG; ALPPL; GCAP; Alkaline phosphatase Regan isozyme; Placental alkaline phosphatase 1; ALPP rtu elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of ALPP. No significant cross-reactivity or interference between ALPP and analogues was observed.
Sequence Length
535
Samples
Serum, plasma, tissue homogenates or other biological fluids
Assay Type
Quantitative Sandwich
Detection Range
12.5-800pg/mL
Sensitivity
< 5.1pg/mL
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level ALPP were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level ALPP were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
Preparation and Storage
For unopened kits: All the reagents should be kept at 4 degree C upon receipt.
For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.
For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for ALPP rtu elisa kit
Intended Uses: The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of ALPP in human serum, plasma, tissue homogenates or other biological fluids.
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to ALPP. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to ALPP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain ALPP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of ALPP in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to ALPP. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to ALPP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain ALPP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of ALPP in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
57,954 Da
NCBI Official Full Name
alkaline phosphatase, placental type preproprotein
NCBI Official Synonym Full Names
alkaline phosphatase, placental
NCBI Official Symbol
ALPP
NCBI Official Synonym Symbols
ALP; PALP; PLAP; PLAP-1
NCBI Protein Information
alkaline phosphatase, placental type
UniProt Protein Name
Alkaline phosphatase, placental type
Protein Family
UniProt Gene Name
ALPP
UniProt Synonym Gene Names
PLAP; PLAP-1
NCBI Description
The protein encoded by this gene is an alkaline phosphatase, a metalloenzyme that catalyzes the hydrolysis of phosphoric acid monoesters. It belongs to a multigene family composed of four alkaline phosphatase isoenzymes. The enzyme functions as a homodimer and has a catalytic site containing one magnesium and two zinc ions, which are required for its enzymatic function. The protein is primarily expressed in placental and endometrial tissue; however, strong ectopic expression has been detected in ovarian adenocarcinoma, serous cystadenocarcinoma, and other ovarian cancer cells. [provided by RefSeq, Jan 2015]
Uniprot Description
MiscellaneousIn most mammals there are four different isozymes: placental, placental-like, intestinal and tissue non-specific (liver/bone/kidney).
Research Articles on ALPP
1. Concentrations of PLAP were elevated in gingival crevicular fluid of patients with pre-eclampsia.
Similar Products
Product Notes
The Human ALPP alpp (Catalog #AAA457560) is a RTU ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA457560 RTU ELISA Kit recognizes Human ALPP. It is sometimes possible for the material contained within the vial of "Alkaline Phosphatase, Placental (ALPP), RTU ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
