Integrin alpha5, beta1 Protein
Integrin alpha5, beta1 (VLA5, Fibronectin Receptor, Very Late Antigen, CD49)
Applications
Western Blot
Purity
Affinity Purified~95%. Non-reduced SDS-PAGE of 15ug on 5-15% polyacrylamide slab gel and staining with Coomassie blue shows only two main bands at 120kD (beta1 subunit) and 145kD (alpha5 subunit). A weak band at 205kD is due to dimerization of the bet
Synonyms
Integrin alpha5; beta1; beta1 (VLA5; Fibronectin Receptor; Very Late Antigen; CD49); beta1 protein
Host
Human
Purity/Purification
Affinity Purified
~95%. Non-reduced SDS-PAGE of 15ug on 5-15% polyacrylamide slab gel and staining with Coomassie blue shows only two main bands at 120kD (beta1 subunit) and 145kD (alpha5 subunit). A weak band at 205kD is due to dimerization of the bet
~95%. Non-reduced SDS-PAGE of 15ug on 5-15% polyacrylamide slab gel and staining with Coomassie blue shows only two main bands at 120kD (beta1 subunit) and 145kD (alpha5 subunit). A weak band at 205kD is due to dimerization of the bet
Form/Format
Supplied as a liquid in 20mM Tris-HCl, pH 7.5, 150mM sodium chloride, 2mM MgCl2, containing 0.2% Triton X-100. No preservatives.
Applicable Applications for Integrin alpha5, beta1 protein
Western Blot (WB)
Application Notes
Suitable for use in Western Blot, Electrophoresis and Functional Studies. In Functional Studies, liposomes containing Integrin alpha5/beta1 adhere to surfaces coated with fibronectin in an RGD-dependent fashion.
Preparation and Storage
Aliquot to avoid repeated freezing and thawing and store at -70 degree C. Aliquots are stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Related Product Information for Integrin alpha5, beta1 protein
The alpha5 beta1 integrin mediates cell adhesion and migration on fibronectin, a glycoprotein critical for normal vertebrate embryonic development. Indirect evidence reported to date suggests that this receptor also functions in the deposition of fibronectin matrices.
Integrin alpha5/beta1 purified from smooth muscle under nondenaturing conditions by affinity chromatography on fibronectin-sepharose.
Integrin alpha5/beta1 purified from smooth muscle under nondenaturing conditions by affinity chromatography on fibronectin-sepharose.
Product Categories/Family for Integrin alpha5, beta1 protein
Similar Products
Product Notes
The Integrin alpha5, beta1 (Catalog #AAA634424) is a Protein produced from Human and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Integrin alpha5, beta1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Suitable for use in Western Blot, Electrophoresis and Functional Studies. In Functional Studies, liposomes containing Integrin alpha5/beta1 adhere to surfaces coated with fibronectin in an RGD-dependent fashion. Researchers should empirically determine the suitability of the Integrin alpha5, beta1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Integrin alpha5, beta1, Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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