Von Willebrand Factor, Factor VIII Free Protein
Von Willebrand Factor, Factor VIII Free, Human (vWF, Factor VIII Related Antigen)
Purity
Highly Purified95% pure by SDS-PAGE under reducing conditions. Consists of large multimers as determined by electrophoresis in SDS/agarose gels.
Synonyms
Von Willebrand Factor; Factor VIII Free; Human (vWF; Factor VIII Related Antigen); Factor VIII Free protein
Host
Human plasma
Purity/Purification
Highly Purified
95% pure by SDS-PAGE under reducing conditions. Consists of large multimers as determined by electrophoresis in SDS/agarose gels.
95% pure by SDS-PAGE under reducing conditions. Consists of large multimers as determined by electrophoresis in SDS/agarose gels.
Form/Format
Supplied as a liquid in 25mM sodium citrate, 100mM glycine, 100mM sodium chloride, pH 6.8.
Mode of Action
Facilitates platelet plug formation by forming a bridge between platelet glycoprotein IB and exposed collagen in the subendothelium. Also binds and transports Factor VIII.
Structure
Multimeric protein composed of identical 250kD molecular weight subunits.
Note
Immediately after removal from dry ice (-80 degree C). Thaw in 37 degree C water bath for several minutes to prevent a precipitation of vWF. Do not agitate or vortex.
Preparation and Storage
Store at -70 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Related Product Information for Von Willebrand Factor, Factor VIII Free protein
Multimeric Structure of von Willebrand Factor: Each vWF monomer (Mr = 260kD) contains a Factor VIII binding site near the NH2-terminal end of the molecule. The monomers are joined end-to-end (NH2 to NH2 and COOH to COOH) by disulfide bonds to form large multimers. The mature multimers can bind one Factor VIII molecule per monomeric subunit.
In addition to its role in platelet plug formation, vWF is also responsible for the binding and transport of Factor VIII (Antihemophilic Aactor) in plasma (15). It appears that this latter event is responsible for both the stability and effective delivery of functional Factor VIII. Studies indicate that Factor VIII binds to the NH2-terminal portion of the mature vWF subunit with a stoichiometry of one Factor VIII molecule per vWF monomer (16,17).
The single chain vWF monomer contains a large number of cysteine residues at both the NH2-terminal and COOH-terminal ends, which are involved in the multimer formation. Carbohydrate analyses indicate that nearly 15% of the mass of vWF is contributed by carbohydrate (18). It appears that the carbohydrate serves to protect vWF from proteolysis, but is not necessary for functional activity or multimer formation.
In addition to its role in platelet plug formation, vWF is also responsible for the binding and transport of Factor VIII (Antihemophilic Aactor) in plasma (15). It appears that this latter event is responsible for both the stability and effective delivery of functional Factor VIII. Studies indicate that Factor VIII binds to the NH2-terminal portion of the mature vWF subunit with a stoichiometry of one Factor VIII molecule per vWF monomer (16,17).
The single chain vWF monomer contains a large number of cysteine residues at both the NH2-terminal and COOH-terminal ends, which are involved in the multimer formation. Carbohydrate analyses indicate that nearly 15% of the mass of vWF is contributed by carbohydrate (18). It appears that the carbohydrate serves to protect vWF from proteolysis, but is not necessary for functional activity or multimer formation.
Product Categories/Family for Von Willebrand Factor, Factor VIII Free protein
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Product Notes
The Von Willebrand Factor, Factor VIII Free (Catalog #AAA635231) is a Protein produced from Human plasma and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Von Willebrand Factor, Factor VIII Free, Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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