Thrombin, beta Protein
Thrombin, beta, Human
Purity
PurifiedHuman b-thrombin is prepared from purified a-thrombin by limited proteolysis with TPCK-treated trypsin, essentially by the method of Braun et al. (5). Purity is assessed by SDS-PAGE and activity is assessed using a fibrinogen clotting assay.
Synonyms
Thrombin; beta; Human; beta protein
Host
Human plasma
Purity/Purification
Purified
Human b-thrombin is prepared from purified a-thrombin by limited proteolysis with TPCK-treated trypsin, essentially by the method of Braun et al. (5). Purity is assessed by SDS-PAGE and activity is assessed using a fibrinogen clotting assay.
Human b-thrombin is prepared from purified a-thrombin by limited proteolysis with TPCK-treated trypsin, essentially by the method of Braun et al. (5). Purity is assessed by SDS-PAGE and activity is assessed using a fibrinogen clotting assay.
Form/Format
The proteolyzed form of alpha thrombin is supplied as a liquid in 100mM sodium phosphate, pH 6.5.
Extinction Coefficient
E1%1cm, 280nm=18.3
Mode of Action
Proteolyzed forms of thrombin which retain activity toward small substrates, factor XIII and prothrombin, but have reduced activity toward fibrinogen, protein C activation and antithrombin III binding.
Structure
Three chains (A, B1, B2), disulfide link between the A and the B2 chains.
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Related Product Information for Thrombin, beta protein
Alpha-thrombin is a highly specific serine protease generated by proteolytic activation of the zymogen prothrombin (1). Purified forms of a-thrombin have been shown (2-4) to undergo autolysis upon long term storage to less active forms. Similar inactivation was observed upon limited digestion of a-thrombin with trypsin (5). These proteolyzed forms of a-thrombin have been termed b-thrombin and g-thrombin. b-thrombin is produced by the cleavage of the Arg-70 or the Arg-73 bond in the thrombin B-chain. Another form of proteolyzed thrombin, termed b'-thrombin is formed by the single cleavage of thrombin at Arg-154. g-Thrombin is produced by proteolytic cleavage at both of these sites (Arg70/73 and Arg-154) in the B-chain. These cleavages cause release of peptides that are no longer covalently attached to the thrombin molecule, but remain associated through ion exchange and gel filtration chromatography. These proteolyzed forms of thrombin retain their ability to cleave small synthetic substrates (6,7) and some protein substrates such as factor XIII (8), antithrombin III (9) and prothrombin (10). Their ability to clot fibrinogen (11), cleave thrombospondin (12) or activate protein C (9) have been markedly decreased.
Product Categories/Family for Thrombin, beta protein
Similar Products
Product Notes
The Thrombin, beta (Catalog #AAA635031) is a Protein produced from Human plasma and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Thrombin, beta, Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
