Protein C, Activated, Dansyl EGR Protein
Protein C, Activated, Dansyl EGR, Human (APC)
Purity
Highly PurifiedActivated protein C is prepared from purified protein C by activation with thrombin followed by ion exchange chromatography (4). Purity is determined by SDS-PAGE analysis.
Synonyms
Protein C; Activated; Dansyl EGR; Human (APC); Dansyl EGR protein
Host
Human plasma
Purity/Purification
Highly Purified
Activated protein C is prepared from purified protein C by activation with thrombin followed by ion exchange chromatography (4). Purity is determined by SDS-PAGE analysis.
Activated protein C is prepared from purified protein C by activation with thrombin followed by ion exchange chromatography (4). Purity is determined by SDS-PAGE analysis.
Form/Format
Supplied as a liquid in 50% glycerol/H2O.
Extinction Coefficient
E1%1cm, 280nm=14.5 (9)
Isoelectric Point
4.4-4.8 (9)
Mode of Action
Anticoagulant, inactivates factors Va and VIIIa
Structure
Two chains, Mr=35,000 and 21,000, disulfide linked, NH2-terminal gla domain two EGF domains
Percent Carbohydrate
23% (5)
Post-translational Modifications
Nine gla residues, one b-hydroxyaspartate
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Related Product Information for Protein C, Activated, Dansyl EGR protein
Inactivation of Factor Va by Activated Protein C (APC): The inactivation of factor Va to form factor Vai results from proteolysis of the factor Va heavy chain (94K) at three specific sites by APC (1,2). The location of these cleavage sites in the factor Va heavy chain are as follows: (human: R306, R506, & R679) and (bovine: R306, R505, & R662). Complete inactivation of the cofactor molecule requires cleavage at the Arginine-306 position. Cleavage at Arginine-306 by activated protein C occurs only in the presence of membrane, and requires prior cleavage of the heavy chain at Arginine-505. Proteolysis of the factor Va light chain by APC occurs only in the bovine molecule and is not required for inactivation.
Activated protein C (APC) is an anticoagulant serine protease derived from the two chain, vitamin K-dependent zymogen, protein C (3-7). A complex between a-thrombin and thrombomodulin catalyzes a single cleavage at Arg-12 (Arg-14 in bovine) in the heavy chain of protein C, to generate APC. Several non-physiologically relevant proteases such as RVV-X activator, trypsin, and PROTAC are also capable of activating protein C.
Activated protein C (APC) is an anticoagulant serine protease derived from the two chain, vitamin K-dependent zymogen, protein C (3-7). A complex between a-thrombin and thrombomodulin catalyzes a single cleavage at Arg-12 (Arg-14 in bovine) in the heavy chain of protein C, to generate APC. Several non-physiologically relevant proteases such as RVV-X activator, trypsin, and PROTAC are also capable of activating protein C.
Product Categories/Family for Protein C, Activated, Dansyl EGR protein
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Product Notes
The Protein C, Activated, Dansyl EGR (Catalog #AAA635043) is a Protein produced from Human plasma and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Protein C, Activated, Dansyl EGR, Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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