Thromboglobulin, beta Peptide
Thromboglobulin, beta, Human
Purity
Affinity PurifiedHuman b-TG is prepared from the supernatant of activated platelets by heparin-agarose affinity chromatography and gel filtration (1,2). Purity is assessed by SDS-PAGE analysis.
Synonyms
Thromboglobulin; beta; Human; beta peptide
Host
Human plasma
Purity/Purification
Affinity Purified
Human b-TG is prepared from the supernatant of activated platelets by heparin-agarose affinity chromatography and gel filtration (1,2). Purity is assessed by SDS-PAGE analysis.
Human b-TG is prepared from the supernatant of activated platelets by heparin-agarose affinity chromatography and gel filtration (1,2). Purity is assessed by SDS-PAGE analysis.
Form/Format
Supplied as a liquid in 20mM Tris, 150mM NaCl, 2mM CaCl2, pH7.4.
Application Notes
Suitable as a marker of platelet activation.
Extinction Coefficient
E1%1cm, 280nm=2.6; Calculated based upon amino acid sequence and molecular weight
Mode of Action
Heparin-binding protein
Structure
Homotetramer (monomer, Mr~8800) (5).
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage, aliquot and store at -20 degree C. Aliquots are stable for at least 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Related Product Information for Thromboglobulin, beta peptide
Domain Structure of b-Thromboglobulin: The domain structure of the b-thromboglobulin monomer is represented. The b-thromboglobulin monomer is an 8,800 molecular weight peptide which is derived via NH2-terminal proteolysis of a precursor molecule LAPF-4 (low affinity platelet factor-4). Although the COOH-terminal domain of b-thromboglobulin is characterized by a clustering of basic lysine residues, the affinity of b-thromboglobulin for heparin is significantly weaker than that of platelet factor-4. In its native state, b-thromboglobulin is a homotetramer consisting of four, identical, noncovalently-associated peptide chains.
The physiological function of b-TG is not known. While early studies suggested that the precursor forms of b-TG were mitogenic for mouse fibroblasts (8,11), it was later concluded that this activity was due to growth factor contamination (10). b-TG has also been reported to inhibit prostacyclin-I2 production by endothelial cells (12), however, the relevance of this effect has been called into question (13,14). The chemotactic activity of platelet a-granule proteins for human fibroblasts has been attributed to both PF-4 and b-TG (15).
The physiological function of b-TG is not known. While early studies suggested that the precursor forms of b-TG were mitogenic for mouse fibroblasts (8,11), it was later concluded that this activity was due to growth factor contamination (10). b-TG has also been reported to inhibit prostacyclin-I2 production by endothelial cells (12), however, the relevance of this effect has been called into question (13,14). The chemotactic activity of platelet a-granule proteins for human fibroblasts has been attributed to both PF-4 and b-TG (15).
Product Categories/Family for Thromboglobulin, beta peptide
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Product Notes
The Thromboglobulin, beta (Catalog #AAA633960) is a Peptide produced from Human plasma and is intended for research purposes only. The product is available for immediate purchase. Suitable as a marker of platelet activation. Researchers should empirically determine the suitability of the Thromboglobulin, beta for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Thromboglobulin, beta, Peptide" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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