Myosin Phospho-Regulation ICC Kit
Myosin Light Chain Phospho-Regulation
Reactivity
Human, Rat, Mouse
Applications
Immunocytochemistry, Immunohistochemistry
Synonyms
Myosin Phospho-Regulation; Myosin Light Chain Phospho-Regulation; Myosin LightK2; Myosin Phospho-Regulation icc kit
Reactivity
Human, Rat, Mouse
Form/Format
Rabbit polyclonals and secondary reagent are supplied inphosphate-buffered saline, 50% glycerol, 1mg/ml BSA, and 0.05% sodium azide.
Applicable Applications for Myosin Phospho-Regulation icc kit
Immunocytochemistry (ICC), Immunohistochemistry (IHC)
Preparation and Storage
Store at -20 degree C. Stable for 1 year.
Related Product Information for Myosin Phospho-Regulation icc kit
Kit Summary: The Myosin Regulatory Light Chain phospho-regulation kitcan be used for immunocytochemical co-localization ofMLC phosphorylation at Ser-1 and Ser-19 compared to thetotal expression of MLC. The kit includes rabbit polyclonalantibodies along with Goat-anti-rabbit conjugated toDyLight 594.
Background: Both smooth muscle and non-muscle myosin II activity is regulated bythe phosphorylation state of the myosin regulatory light chain (MLC, MRLC, MLC20, Myl9). Phosphorylation of MLC at Thr-18 and Ser-19activates myosin II motor activity and increases myosin filamentstability. This activation has important roles in various cell motileprocesses. By contrast, other phosphorylation sites on MLC may inhibitmyosin II activity. PKC phosphorylates Ser-1/Ser-2 and Thr-9 in MLC, and this phosphorylation decreases activated myosin II interaction withactin, as well as inhibits MLC interaction with the activation site kinase, myosin light-chain kinase. The Ser-1/Ser-2 region may be the majorinhibitory site since Ser-1 is phosphorylated during PDGF-inducedstress fiber disassembly and expression of unphosphoryla table MLC20 at the Ser-1/Ser-2 site suppresses this disassembly. Thus, inhibition ofmyosin II activity through phosphorylation of Ser-1/Ser-2 may have important roles in growth factor-induced reorganization of actomyosin filaments.
Background: Both smooth muscle and non-muscle myosin II activity is regulated bythe phosphorylation state of the myosin regulatory light chain (MLC, MRLC, MLC20, Myl9). Phosphorylation of MLC at Thr-18 and Ser-19activates myosin II motor activity and increases myosin filamentstability. This activation has important roles in various cell motileprocesses. By contrast, other phosphorylation sites on MLC may inhibitmyosin II activity. PKC phosphorylates Ser-1/Ser-2 and Thr-9 in MLC, and this phosphorylation decreases activated myosin II interaction withactin, as well as inhibits MLC interaction with the activation site kinase, myosin light-chain kinase. The Ser-1/Ser-2 region may be the majorinhibitory site since Ser-1 is phosphorylated during PDGF-inducedstress fiber disassembly and expression of unphosphoryla table MLC20 at the Ser-1/Ser-2 site suppresses this disassembly. Thus, inhibition ofmyosin II activity through phosphorylation of Ser-1/Ser-2 may have important roles in growth factor-induced reorganization of actomyosin filaments.
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Product Notes
The Human, Rat, Mouse Myosin Phospho-Regulation (Catalog #AAA474429) is an ICC Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA474429 ICC Kit recognizes Human, Rat, Mouse Myosin Phospho-Regulation. AAA Biotech's Myosin Phospho-Regulation can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunohistochemistry (IHC). Researchers should empirically determine the suitability of the Myosin Phospho-Regulation for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Myosin Phospho-Regulation, ICC Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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