Tuberin/TSC2 ELISA Kit | TSC2 elisa kit

Phospho-Tuberin/TSC2 (Ser939) Colorimetric Cell-Based ELISA Kit

Cat. Num. AAA9501169

• Gene Names: TSC2; LAM; TSC4; PPP1R160
• Reactivity: Human, Mouse, Rat
• Synonyms: Tuberin/TSC2; Phospho-Tuberin/TSC2 (Ser939) Colorimetric Cell-Based ELISA Kit; TSC2; tuberous sclerosis 2 gene; Tuberous sclerosis 2 homolog protein; Tuberous sclerosis 2 protein; TSC2 elisa kit

• Reactivity: Human, Mouse, Rat
• Form/Format: Two 96-Well Plates
• Sequence Length: 1807

• Preparation and Storage: Store at 4 degree C.

Related Product Information for TSC2 elisa kit

Background/Introduction: The Colorimetric Cell-Based ELISA Kit allows for the detection of various target proteins and the effects that certain stimulation conditions have on target protein expression in different cell lines. Qualitative determination of target protein concentration is achieved by an indirect ELISA format. In essence, the target protein is captured by target-specific primary (1 degree) antibodies while the HRP-conjugated secondary (2 degree) antibodies bind the Fc region of the 1 degree antibody. Through this binding, the HRP enzyme conjugated to the 2 degree antibody can catalyze a colorimetric reaction upon substrate addition. Due to the qualitative nature of the Cell-Based ELISA, multiple normalization methods are described: 1) a monoclonal antibody specific for human GAPDH is included to serve as an internal positive control in normalizing the target absorbance values. 2) Following the colorimetric measurement of HRP activity via substrate addition, the Crystal Violet whole-cell staining method is used to determine cell density. After staining, the results can be analyzed by normalizing the absorbance values to cell amounts, by which the plating difference can be adjusted. 3) If a phosphorylated target is being detected, an antibody against the non- phosphorylated counterpart will be provided for normalization purposes. The absorbance values obtained for the non-phosphorylated target can be used to normalize the absorbance values for the phosphorylated target.

Product Categories/Family for TSC2 elisa kit

Cell Based ELISA Kit

NCBI and Uniprot Product Information

• NCBI GI #: 116256352
• NCBI GeneID: 7249
• NCBI Accession #: NP_000539.2
• NCBI GenBank Nucleotide #: NM_000548.4
• UniProt Accession #: P49815; O75275; Q4LE71; Q8TAZ1; A7E2E2; B4DIL8; B4DIQ7; B4DRN2; B7Z2B8; C9J378
• Molecular Weight: 200749
• NCBI Official Full Name: tuberin isoform 1
• NCBI Official Synonym Full Names: TSC complex subunit 2
• NCBI Official Symbol: TSC2
• NCBI Official Synonym Symbols: LAM; TSC4; PPP1R160
• NCBI Protein Information: tuberin
• UniProt Protein Name: Tuberin
• Protein Family: Tuberin
• UniProt Gene Name: TSC2
• UniProt Synonym Gene Names: TSC4

NCBI Description

Mutations in this gene lead to tuberous sclerosis complex. Its gene product is believed to be a tumor suppressor and is able to stimulate specific GTPases. The protein associates with hamartin in a cytosolic complex, possibly acting as a chaperone for hamartin. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]

Uniprot Description

TSC2: a product of the tumor suppressor gene TSC2. Tuberin and Hamartin (TSC1) form a tumor suppressor heterodimer that inhibits the mTOR nutrient signaling input. TSC1/TSC2 targets the small G protein Rheb, a novel mediator of the nutrient signaling input to mTOR. Functions as a Rheb GTPase activating protein (GAP). Four alternatively spliced isoforms have been described.

Protein type: Cell cycle regulation; GAP; GAP, misc.; Nuclear receptor co-regulator; Tumor suppressor

Chromosomal Location of Human Ortholog: 16p13.3

Cellular Component: cytoplasm; cytosol; Golgi apparatus; lysosome; membrane; nucleus; perinuclear region of cytoplasm; TSC1-TSC2 complex

Molecular Function: GTPase activator activity; phosphatase binding; protein binding; protein homodimerization activity; small GTPase binding

Biological Process: anoikis; cell cycle arrest; endocytosis; heart development; insulin-like growth factor receptor signaling pathway; negative regulation of cell proliferation; negative regulation of insulin receptor signaling pathway; negative regulation of mitophagy; negative regulation of phosphatidylinositol 3-kinase signaling; negative regulation of protein kinase activity; negative regulation of protein kinase B signaling cascade; negative regulation of TOR signaling; negative regulation of Wnt signaling pathway; neural tube closure; positive chemotaxis; positive regulation of GTPase activity; positive regulation of macroautophagy; protein import into nucleus; protein kinase B signaling; protein localization; regulation of cell cycle; regulation of endocytosis; regulation of insulin receptor signaling pathway; regulation of small GTPase mediated signal transduction; vesicle-mediated transport; viral process

Disease: Focal Cortical Dysplasia Of Taylor; Lymphangioleiomyomatosis; Tuberous Sclerosis 2