Testing Data
(The TFact Smad2 (Phospho-Ser250) DNA-Binding ELISA detects active Smad2 (Phospho-Ser250) in Jurkat Nuclear Extract. The Jurkat cells were grown 3 days in RPMI 1640 with 10% FBS and harvested for nuclear extract.)
SMAD2 elisa kit
Smad2 (Phospho-Ser250) TFact DNA-Binding ELISA Kit
Testing Data
(The TFact Smad2 (Phospho-Ser250) DNA-Binding ELISA detects active Smad2 (Phospho-Ser250) in Jurkat Nuclear Extract. The Jurkat cells were grown 3 days in RPMI 1640 with 10% FBS and harvested for nuclear extract.)
Testing Data
(The TFact Smad2 (Phospho-Ser250) DNA-Binding ELISA detects active Smad2 (Phospho-Ser250) in Jurkat Nuclear Extract. The Jurkat cells were grown 3 days in RPMI 1640 with 10% FBS and harvested for nuclear extract.)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signal of the transforming growth factor (TGF)-beta, and thus regulates multiple cellular processes, such as cell proliferation, apoptosis, and differentiation. This protein is recruited to the TGF-beta receptors through its interaction with the SMAD anchor for receptor activation (SARA) protein. In response to TGF-beta signal, this protein is phosphorylated by the TGF-beta receptors. The phosphorylation induces the dissociation of this protein with SARA and the association with the family member SMAD4. The association with SMAD4 is important for the translocation of this protein into the nucleus, where it binds to target promoters and forms a transcription repressor complex with other cofactors. This protein can also be phosphorylated by activin type 1 receptor kinase, and mediates the signal from the activin. Alternatively spliced transcript variants have been observed for this gene. [provided by RefSeq, May 2012]
Uniprot Description
SMAD2: ubiquitously expressed transcription factor phosphorylated and activated by TGF-beta receptor-type kinases. Participates in a wide range of critical processes including morphogenesis, cell-fate determination, proliferation, differentiation and apoptosis. Phosphorylated Smads dimerize with collaborating Smad4 and are translocated into the nucleus, where the transcription of target genes is stimulated. Two alternatively spliced isoforms have been described.
Protein type: DNA-binding; Transcription factor
Chromosomal Location of Human Ortholog: 18q21.1
Cellular Component: cytoplasm; cytosol; nuclear chromatin; nucleoplasm; nucleus; protein complex; SMAD protein complex; SMAD2-SMAD3 protein complex; transcription factor complex
Molecular Function: activating transcription factor binding; chromatin binding; co-SMAD binding; DNA binding; DNA binding transcription factor activity; double-stranded DNA binding; I-SMAD binding; metal ion binding; phosphatase binding; protein binding; protein heterodimerization activity; protein homodimerization activity; R-SMAD binding; SMAD binding; transcription factor binding; transforming growth factor beta receptor binding; transforming growth factor beta receptor, pathway-specific cytoplasmic mediator activity; type I transforming growth factor beta receptor binding; ubiquitin protein ligase binding
Biological Process: activin receptor signaling pathway; anterior/posterior pattern specification; cell fate commitment; common-partner SMAD protein phosphorylation; embryonic cranial skeleton morphogenesis; embryonic foregut morphogenesis; endoderm formation; gastrulation; in utero embryonic development; insulin secretion; lung development; mesoderm formation; negative regulation of cell proliferation; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; negative regulation of transforming growth factor beta receptor signaling pathway; nodal signaling pathway; organ growth; palate development; pancreas development; paraxial mesoderm morphogenesis; pericardium development; positive regulation of BMP signaling pathway; positive regulation of epithelial to mesenchymal transition; positive regulation of nodal signaling pathway involved in determination of lateral mesoderm left/right asymmetry; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-templated; post-embryonic development; primary microRNA processing; protein deubiquitination; regulation of binding; regulation of transforming growth factor beta receptor signaling pathway; response to glucose stimulus; SMAD protein complex assembly; somatic stem cell maintenance; transcription from RNA polymerase II promoter; transforming growth factor beta receptor signaling pathway; ureteric bud development; wound healing; zygotic determination of dorsal/ventral axis
Research Articles on SMAD2
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Product Notes
The Human, Mouse, Rat SMAD2 smad2 (Catalog #AAA9502245) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9502245 ELISA Kit recognizes Human, Mouse, Rat SMAD2. It is sometimes possible for the material contained within the vial of "Smad2, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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