p90 RSK elisa kit

Phospho-p90 RSK (Thr359+Ser363) Fluorometric Cell-Based ELISA Kit

Cat. Num. AAA9501756

• Gene Names: RPS6KA1; RSK; HU-1; RSK1; p90Rsk; MAPKAPK1A
• Reactivity: Human, Mouse, Rat
• Synonyms: p90 RSK; Phospho-p90 RSK (Thr359+Ser363) Fluorometric Cell-Based ELISA Kit; 90 kDa ribosomal protein S6 kinase 1; kinase p90RSK1; KS6A1; KS6AA; MAPKAP-K1a; p90-RSK 1; pp90RSK1; Ribosomal protein S6 kinase alpha 1; Ribosomal S6 kinase 1; RPS6KA1; RSK-1; RSK1; S6K-alpha 1; p90 RSK elisa kit

• Reactivity: Human, Mouse, Rat
• Form/Format: Two 96-Well Plates
• Sequence Length: 744

• Preparation and Storage: Store at 4 degree C.

Related Product Information for p90 RSK elisa kit

Background/Introduction: The Fluorometric Cell-Based ELISA Kit allows for the detection of various target proteins and the effects that certain stimulation conditions have on target protein expression in different cell lines. Qualitative determination of target protein concentration is achieved by an indirect ELISA format. In essence, the target protein is captured by target-specific primary (1 degree) antibodies while Dye 1-conjugated and Dye 2-conjugated secondary (2 degree) antibodies bind the Fc region of the 1 degree antibody. Through this binding, the dye conjugated to the 2 degree antibody can emit light at a certain wavelength given proper excitation, hence allowing for a fluorometric detection method. A monoclonal antibody specific for human GAPDH is included to serve as an internal positive control in normalizing the target RFU values. If a phosphorylated target is being detected, an antibody against the non-phosphorylated counterpart will be provided for normalization purposes. The RFU values obtained for the non-phosphorylated target can be used to normalize the RFU value for the phosphorylated target.

NCBI and Uniprot Product Information

• NCBI GI #: 55743134
• NCBI GeneID: 6195
• NCBI Accession #: NP_001006666.1
• NCBI GenBank Nucleotide #: NM_001006665.1
• UniProt Accession #: Q15418; Q5SVM5; Q5SVM8; Q5SVM9; Q96C05; Q9BQK2; A6NGG4; A8K9K7; B2RDY8; B7Z5J0; E9PRI4
• Molecular Weight: 82723
• NCBI Official Full Name: ribosomal protein S6 kinase alpha-1 isoform b
• NCBI Official Synonym Full Names: ribosomal protein S6 kinase A1
• NCBI Official Symbol: RPS6KA1
• NCBI Official Synonym Symbols: RSK; HU-1; RSK1; p90Rsk; MAPKAPK1A
• NCBI Protein Information: ribosomal protein S6 kinase alpha-1
• UniProt Protein Name: Ribosomal protein S6 kinase alpha-1
• UniProt Gene Name: RPS6KA1
• UniProt Synonym Gene Names: MAPKAPK1A; RSK1; S6K-alpha-1; p90-RSK 1; p90RSK1; p90S6K; MAPK-activated protein kinase 1a; MAPKAP kinase 1a; MAPKAPK-1a; RSK-1

NCBI Description

This gene encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 nonidentical kinase catalytic domains and phosphorylates various substrates, including members of the mitogen-activated kinase (MAPK) signalling pathway. The activity of this protein has been implicated in controlling cell growth and differentiation. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]

Uniprot Description

p90RSK: an AGC kinase of the RSK family. Phosphorylated and activated by Erk1 and -2 in response to many growth factors, polypeptide hormones and neurotransmitters. Several phosphorylation sites are important for its activation. Possesses two kinase domains connected by a regulator linker region. Phosphorylates a wide range of substrates including ribosomal protein S6. Prominently expressed in brain structures essential for cognitive function and learning.

Protein type: AGC group; EC 2.7.11.1; Kinase, protein; Protein kinase, AGC; Protein kinase, Ser/Thr (non-receptor); RSK family; RSK subfamily; Translation

Chromosomal Location of Human Ortholog: 1p36.11

Cellular Component: cytoplasm; cytosol; nucleoplasm; nucleus

Molecular Function: ATP binding; caspase inhibitor activity; kinase activity; magnesium ion binding; protein binding; protein serine/threonine kinase activity; protein serine/threonine/tyrosine kinase activity

Biological Process: apoptosis; cell cycle; hepatocyte proliferation; negative regulation of apoptosis; negative regulation of cysteine-type endopeptidase activity involved in apoptotic process; positive regulation of cell differentiation; positive regulation of cell growth; positive regulation of hepatic stellate cell activation; positive regulation of transcription from RNA polymerase II promoter; protein amino acid phosphorylation; regulation of DNA-templated transcription in response to stress; regulation of translation in response to stress; signal transduction