p300 elisa kit

p300 Colorimetric Cell-Based ELISA Kit

Cat. Num. AAA9500441

• Gene Names: EP300; p300; KAT3B; RSTS2
• Reactivity: Human, Mouse, Rat
• Synonyms: p300; p300 Colorimetric Cell-Based ELISA Kit; E1A-associated protein p300; EP300; P300 transcription coactivator homolog; p300 elisa kit

• Reactivity: Human, Mouse, Rat
• Form/Format: One 96-Well Plate
• Sequence Length: 2414

• Samples: Researchers plate their cell line of choice
• Assay Type: Non-Phospho Cell-Based ELISA
• Sensitivity: >=5000 cells/well
• Detection Method: Colorimetric 450 nm
• Preparation and Storage: Store at 4 degree C.

Product Categories/Family for p300 elisa kit

Cell Based ELISA Kit

NCBI and Uniprot Product Information

• NCBI GI #: 50345997
• NCBI GeneID: 2033
• NCBI Accession #: NP_001420.2
• NCBI GenBank Nucleotide #: NM_001429.3
• UniProt Accession #: Q09472; B1AKC2
• Molecular Weight: 264144
• NCBI Official Full Name: histone acetyltransferase p300
• NCBI Official Synonym Full Names: E1A binding protein p300
• NCBI Official Symbol: EP300
• NCBI Official Synonym Symbols: p300; KAT3B; RSTS2
• NCBI Protein Information: histone acetyltransferase p300
• UniProt Protein Name: Histone acetyltransferase p300
• UniProt Gene Name: EP300
• UniProt Synonym Gene Names: P300; p300 HAT

NCBI Description

This gene encodes the adenovirus E1A-associated cellular p300 transcriptional co-activator protein. It functions as histone acetyltransferase that regulates transcription via chromatin remodeling and is important in the processes of cell proliferation and differentiation. It mediates cAMP-gene regulation by binding specifically to phosphorylated CREB protein. This gene has also been identified as a co-activator of HIF1A (hypoxia-inducible factor 1 alpha), and thus plays a role in the stimulation of hypoxia-induced genes such as VEGF. Defects in this gene are a cause of Rubinstein-Taybi syndrome and may also play a role in epithelial cancer. [provided by RefSeq, Jul 2008]

Uniprot Description

p300: a histone acetyltransferase and transcriptional co-activator that regulates transcription via chromatin remodeling. Acetylates all four core histones in nucleosomes. Related to CPB (CREB-binding protein), and like CPB can stimulate transcription through activation of CREB. Specifically inhibited by the adenovirus oncoprotein E1A. A co-activator of HIF1A (hypoxia-inducible factor 1 alpha), and thus plays a role in the stimulation of hypoxia-induced genes such as VEGF. Mediates cAMP-gene regulation by binding specifically to phosphorylated CREB protein. Methylated at R580 and R604 in the KIX domain by CARM1, which blocks association with CREB, inhibits CREB signaling and activates the apoptotic response. Also methylated at R2142 by CARM1, which impairs interaction with NCoA2.

Protein type: Acetyltransferase; EC 2.3.1.48; Motility/polarity/chemotaxis; Nuclear receptor co-regulator; Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 22q13.2

Cellular Component: chromosome; cytosol; histone acetyltransferase complex; nucleoplasm; nucleus; transcription factor complex

Molecular Function: acetyltransferase activity; activating transcription factor binding; androgen receptor binding; beta-catenin binding; chromatin binding; chromatin DNA binding; damaged DNA binding; DNA binding; histone acetyltransferase activity; histone butyryltransferase activity; histone crotonyltransferase activity; lysine N-acetyltransferase activity; nuclear hormone receptor binding; p53 binding; peptide butyryltransferase activity; peptide N-acetyltransferase activity; peptide-lysine-N-acetyltransferase activity; protein binding; protein C-terminus binding; protein propionyltransferase activity; transcription coactivator activity; transcription factor binding; transferase activity, transferring acyl groups; zinc ion binding

Biological Process: apoptosis; B cell differentiation; beta-catenin-TCF complex assembly; cellular response to UV; circadian rhythm; DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; fat cell differentiation; heart development; histone acetylation; internal peptidyl-lysine acetylation; internal protein amino acid acetylation; intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator; lung development; macrophage derived foam cell differentiation; N-terminal peptidyl-lysine acetylation; negative regulation of transcription from RNA polymerase II promoter; nervous system development; Notch signaling pathway; organ morphogenesis; peptidyl-lysine butyrylation; peptidyl-lysine crotonylation; peptidyl-lysine propionylation; platelet formation; positive regulation of gene expression, epigenetic; positive regulation of interferon type I production; positive regulation of protein binding; positive regulation of transcription factor activity; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription of target genes involved in unfolded protein response; protein amino acid acetylation; protein destabilization; protein deubiquitination; protein stabilization; regulation of autophagy; regulation of cell cycle; regulation of cellular response to heat; regulation of megakaryocyte differentiation; regulation of transcription, DNA-templated; regulation of tubulin deacetylation; response to estrogen; response to hypoxia; skeletal muscle tissue development; somitogenesis; stimulatory C-type lectin receptor signaling pathway; transcription from RNA polymerase II promoter; transcription-coupled nucleotide-excision repair; viral process

Disease: Colorectal Cancer; Rubinstein-taybi Syndrome 1; Rubinstein-taybi Syndrome 2