IRS-1 elisa kit

Phospho-IRS-1 (Ser312) Colorimetric Cell-Based ELISA Kit

Cat. Num. AAA9501484

• Gene Names: IRS1; HIRS-1
• Reactivity: Human, Mouse, Rat
• Synonyms: IRS-1; Phospho-IRS-1 (Ser312) Colorimetric Cell-Based ELISA Kit; Insulin receptor substrate-1; IRS1; IRS-1 elisa kit

• Reactivity: Human, Mouse, Rat
• Specificity: The Anti-IRS-1 (Phospho-Ser312) Antibody is a rabbit polyclonal antibody. It was tested on Western Blots for specificity. The data in Figure 2 shows that a single protein band was detected. This protein band can be blocked by the synthesized immunogen peptide.
• Form/Format: Two 96-Well Plates
• Sequence Length: 1242

• Preparation and Storage: Store at 4 degree C.

Related Product Information for IRS-1 elisa kit

Principle of the Assay: The IRS-1 (Phospho-Ser312) Cell-Based ELISA Kit is a convenient, lysate- free, high throughput and sensitive assay kit that can monitor IRS-1 protein phosphorylation and expression profile in cells. The kit can be used for measuring the relative amounts of phosphorylated IRS-1 in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on IRS-1 phosphorylation.

Background/Introduction: The Colorimetric Cell-Based ELISA Kit allows for the detection of various target proteins and the effects that certain stimulation conditions have on target protein expression in different cell lines. Qualitative determination of target protein concentration is achieved by an indirect ELISA format. In essence, the target protein is captured by target-specific primary (1 degree) antibodies while the HRP-conjugated secondary (2 degree) antibodies bind the Fc region of the 1 degree antibody. Through this binding, the HRP enzyme conjugated to the 2 degree antibody can catalyze a colorimetric reaction upon substrate addition. Due to the qualitative nature of the Cell-Based ELISA, multiple normalization methods are described: 1) a monoclonal antibody specific for human GAPDH is included to serve as an internal positive control in normalizing the target absorbance values. 2) Following the colorimetric measurement of HRP activity via substrate addition, the Crystal Violet whole-cell staining method is used to determine cell density. After staining, the results can be analyzed by normalizing the absorbance values to cell amounts, by which the plating difference can be adjusted. 3) If a phosphorylated target is being detected, an antibody against the non- phosphorylated counterpart will be provided for normalization purposes. The absorbance values obtained for the non-phosphorylated target can be used to normalize the absorbance values for the phosphorylated target.

Product Categories/Family for IRS-1 elisa kit

Cell Based ELISA Kit

NCBI and Uniprot Product Information

• NCBI GI #: 5031805
• NCBI GeneID: 3667
• NCBI Accession #: NP_005535.1
• NCBI GenBank Nucleotide #: NM_005544.2
• UniProt Accession #: P35568
• Molecular Weight: 131591
• NCBI Official Full Name: insulin receptor substrate 1
• NCBI Official Synonym Full Names: insulin receptor substrate 1
• NCBI Official Symbol: IRS1
• NCBI Official Synonym Symbols: HIRS-1
• NCBI Protein Information: insulin receptor substrate 1
• UniProt Protein Name: Insulin receptor substrate 1
• UniProt Gene Name: IRS1
• UniProt Synonym Gene Names: IRS-1

NCBI Description

This gene encodes a protein which is phosphorylated by insulin receptor tyrosine kinase. Mutations in this gene are associated with type II diabetes and susceptibility to insulin resistance. [provided by RefSeq, Nov 2009]

Uniprot Description

IRS1: an adaptor protein that is one of the major substrates of the insulin receptor kinase. Contains multiple tyrosine phosphorylation motifs that serve as docking sites for SH2-domain-containing proteins including phosphatidylinositol 3-kinase p85 subunit and GRB-2. Phosphorylated on ser/thr residues by kinases including JNK and IKK.

Protein type: Adaptor/scaffold; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 2q36.3

Cellular Component: caveola; cytoplasm; cytosol; insulin receptor complex; intracellular membrane-bound organelle; nucleus; plasma membrane

Molecular Function: 1-phosphatidylinositol-3-kinase activity; insulin receptor binding; insulin-like growth factor receptor binding; phosphatidylinositol-4,5-bisphosphate 3-kinase activity; phosphoinositide 3-kinase binding; phosphotyrosine binding; protein binding; protein kinase C binding; Ras guanyl-nucleotide exchange factor activity; SH2 domain binding; signal transducer activity; transmembrane receptor protein tyrosine kinase adaptor protein activity

Biological Process: cellular response to insulin stimulus; glucose homeostasis; insulin receptor signaling pathway; insulin-like growth factor receptor signaling pathway; interleukin-7-mediated signaling pathway; MAPK cascade; negative regulation of insulin receptor signaling pathway; negative regulation of insulin secretion; phosphatidylinositol phosphorylation; phosphoinositide 3-kinase cascade; phosphoinositide-mediated signaling; positive regulation of cell proliferation; positive regulation of fatty acid beta-oxidation; positive regulation of glucose import; positive regulation of glucose import in response to insulin stimulus; positive regulation of glucose metabolic process; positive regulation of glycogen biosynthetic process; positive regulation of insulin receptor signaling pathway; positive regulation of phosphatidylinositol 3-kinase activity; positive regulation of protein kinase B signaling; response to insulin stimulus; response to peptide hormone; signal transduction

Disease: Diabetes Mellitus, Noninsulin-dependent