AKAP10 elisa kit

AKAP10 Colorimetric Cell-Based ELISA Kit

Cat. Num. AAA9500664

• Gene Names: AKAP10; PRKA10; AKAP-10; D-AKAP2; D-AKAP-2
• Reactivity: Human, Mouse, Rat
• Synonyms: AKAP10; AKAP10 Colorimetric Cell-Based ELISA Kit; A kinase anchor protein 10; mitochondrial; Protein kinase A-anchoring protein 10; PRKA10; Dual specificity A kinase-anchoring protein 2; D-AKAP-2; AKAP10 elisa kit

• Reactivity: Human, Mouse, Rat
• Form/Format: One 96-Well Plate
• Sequence Length: 662

• Samples: Researchers plate their cell line of choice
• Assay Type: Non-Phospho Cell-Based ELISA
• Sensitivity: >=5000 cells/well
• Detection Method: Colorimetric 450 nm
• Preparation and Storage: Store at 4 degree C.

Product Categories/Family for AKAP10 elisa kit

Cell Based ELISA Kit

NCBI and Uniprot Product Information

• NCBI GI #: 21493033
• NCBI GeneID: 11216
• NCBI Accession #: NP_009133.2
• NCBI GenBank Nucleotide #: NM_007202.3
• UniProt Accession #: O43572; Q96AJ7; B2R650
• Molecular Weight: 73 kDa
• NCBI Official Full Name: A-kinase anchor protein 10, mitochondrial isoform 1
• NCBI Official Synonym Full Names: A-kinase anchoring protein 10
• NCBI Official Symbol: AKAP10
• NCBI Official Synonym Symbols: PRKA10; AKAP-10; D-AKAP2; D-AKAP-2
• NCBI Protein Information: A-kinase anchor protein 10, mitochondrial
• UniProt Protein Name: A-kinase anchor protein 10, mitochondrial
• Protein Family: A-kinase anchor protein
• UniProt Gene Name: AKAP10
• UniProt Synonym Gene Names: AKAP-10; D-AKAP-2; PRKA10

NCBI Description

This gene encodes a member of the A-kinase anchor protein family. A-kinase anchor proteins bind to the regulatory subunits of protein kinase A (PKA) and confine the holoenzyme to discrete locations within the cell. The encoded protein is localized to mitochondria and interacts with both the type I and type II regulatory subunits of PKA. Polymorphisms in this gene may be associated with increased risk of arrhythmias and sudden cardiac death. [provided by RefSeq, May 2012]

Uniprot Description

AKAP10: Differentially targeted protein that binds to type I and II regulatory subunits of protein kinase A and anchors them to the mitochondria or the plasma membrane. Although the physiological relevance between PKA and AKAPS with mitochondria is not fully understood, one idea is that BAD, a proapoptotic member, is phosphorylated and inactivated by mitochondria-anchored PKA. It cannot be excluded too that it may facilitate PKA as well as G protein signal transduction, by acting as an adapter for assembling multiprotein complexes. With its RGS domain, it could lead to the interaction to G-alpha proteins, providing a link between the signaling machinery and the downstream kinase. Genetic variations in AKAP10 are a cause of susceptibility to sudden cardiac death (SCD). Unexpected rapid natural death due to cardiovascular collapse within one hour of initial symptoms. It is usually caused by the worsening of existing heart diseases. The sudden onset of symptoms, such as chest pain and cardiac arrhythmias, particularly ventricular tachycardia, can lead to the loss of consciousness and cardiac arrest followed by biological death. Increased susceptibility to sudden cardiac death may be conferred by AKAP10 variants that are associated with markers of low vagus nerve sensitivity, e.g. fast basal heart rate and low heart rate variability.

Protein type: Adaptor/scaffold

Chromosomal Location of Human Ortholog: 17p11.2

Cellular Component: cytosol; mitochondrion; plasma membrane; protein complex

Molecular Function: protein kinase A binding

Biological Process: blood coagulation; protein localization; signal transduction

Disease: Cardiac Conduction Defect