ACC1 elisa kit

ACC1 Colorimetric Cell-Based ELISA Kit

Cat. Num. AAA9500013

• Gene Names: ACACA; ACC; ACAC; ACC1; ACCA; ACACAD
• Reactivity: Human, Mouse, Rat
• Synonyms: ACC1; ACC1 Colorimetric Cell-Based ELISA Kit; ACAC; ACACA; ACC-alpha; ACCA; Acetyl-CoA carboxylase 1; ACC1 elisa kit

• Reactivity: Human, Mouse, Rat
• Specificity: The Anti-ACC1 Antibody is a rabbit polyclonal antibody. It was tested on Western Blots for specificity. The data in Figure 2 shows that a single protein band was detected. This protein band can be blocked by the synthesized immunogen peptide.
• Form/Format: One 96-Well Plate
• Sequence Length: 2383

• Preparation and Storage: Store at 4 degree C.

Related Product Information for ACC1 elisa kit

Background/Introduction: The Colorimetric Cell-Based ELISA Kit allows for the detection of various target proteins and the effects that certain stimulation conditions have on target protein expression in different cell lines. Qualitative determination of target protein concentration is achieved by an indirect ELISA format. In essence, the target protein is captured by target-specific primary (1 degree) antibodies while the HRP-conjugated secondary (2 degree) antibodies bind the Fc region of the 1 degree antibody. Through this binding, the HRP enzyme conjugated to the 2 degree antibody can catalyze a colorimetric reaction upon substrate addition. Due to the qualitative nature of the Cell-Based ELISA, multiple normalization methods are described: 1) a monoclonal antibody specific for human GAPDH is included to serve as an internal positive control in normalizing the target absorbance values. 2) Following the colorimetric measurement of HRP activity via substrate addition, the Crystal Violet whole-cell staining method is used to determine cell density. After staining, the results can be analyzed by normalizing the absorbance values to cell amounts, by which the plating difference can be adjusted. 3) If a phosphorylated target is being detected, an antibody against the non-phosphorylated counterpart will be provided for normalization purposes. The absorbance values obtained for the non-phosphorylated target can be used to normalize the absorbance values for the phosphorylated target.

Product Categories/Family for ACC1 elisa kit

Cell Based ELISA Kit

NCBI and Uniprot Product Information

• NCBI GI #: 38679960
• NCBI GeneID: 31
• NCBI Accession #: NP_942131.1
• NCBI GenBank Nucleotide #: NM_198834.2
• UniProt Accession #: Q13085; Q6KEV6; Q6XDA8; Q7Z2G8; Q7Z561; Q7Z563; Q7Z564; Q86WB2; Q86WB3; B2RP68
• Molecular Weight: 265554
• NCBI Official Full Name: acetyl-CoA carboxylase 1 isoform 1
• NCBI Official Synonym Full Names: acetyl-CoA carboxylase alpha
• NCBI Official Symbol: ACACA
• NCBI Official Synonym Symbols: ACC; ACAC; ACC1; ACCA; ACACAD
• NCBI Protein Information: acetyl-CoA carboxylase 1
• UniProt Protein Name: Acetyl-CoA carboxylase 1
• Protein Family: Acetyl-CoA carboxylase
• UniProt Gene Name: ACACA
• UniProt Synonym Gene Names: ACAC; ACC1; ACCA; ACC1

NCBI Description

Acetyl-CoA carboxylase (ACC) is a complex multifunctional enzyme system. ACC is a biotin-containing enzyme which catalyzes the carboxylation of acetyl-CoA to malonyl-CoA, the rate-limiting step in fatty acid synthesis. There are two ACC forms, alpha and beta, encoded by two different genes. ACC-alpha is highly enriched in lipogenic tissues. The enzyme is under long term control at the transcriptional and translational levels and under short term regulation by the phosphorylation/dephosphorylation of targeted serine residues and by allosteric transformation by citrate or palmitoyl-CoA. Multiple alternatively spliced transcript variants divergent in the 5' sequence and encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

ACC1: a subunit of acetyl-CoA carboxylase (ACC), a multifunctional enzyme system. Catalyzes the carboxylation of acetyl-CoA to malonyl-CoA, the rate-limiting step in fatty acid synthesis. Phosphorylation by AMPK or PKA inhibits the enzymatic activity of ACC. ACC-alpha is the predominant isoform in liver, adipocyte and mammary gland. ACC-beta is the major isoform in skeletal muscle and heart. Phosphorylation regulates its activity.

Protein type: Carbohydrate Metabolism - propanoate; Carbohydrate Metabolism - pyruvate; EC 6.3.4.14; EC 6.4.1.2; Ligase; Lipid Metabolism - fatty acid biosynthesis

Chromosomal Location of Human Ortholog: 17q12

Cellular Component: actin cytoskeleton; cytosol; extracellular exosome; fibrillar center; mitochondrion

Molecular Function: acetyl-CoA carboxylase activity; ATP binding; biotin carboxylase activity; identical protein binding; metal ion binding; protein binding

Biological Process: acetyl-CoA metabolic process; biotin metabolic process; carnitine shuttle; cellular response to prostaglandin E stimulus; fatty acid biosynthetic process; fatty-acyl-CoA biosynthetic process; lipid homeostasis; malonyl-CoA biosynthetic process; multicellular organismal protein metabolic process; positive regulation of cellular metabolic process; protein homotetramerization; regulation of cholesterol biosynthetic process; tissue homeostasis

Disease: Acetyl-coa Carboxylase Deficiency