Testing Data
(P-peptide-+)
PLD2 Antibody | anti-PLD2 antibody
PLD2 (Phospho-Tyr169) Antibody
Gene Names
PLD2; PLD1C
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, ELISA
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Synonyms
PLD2; PLD2 (Phospho-Tyr169) Antibody; choline phosphatase 2; EC 3.1.4.4; phosphatidylcholine-hydrolyzing phospholipase D2; phospholipase D2; PLD 2; PLD1C; anti-PLD2 antibody
Reactivity
Human, Mouse, Rat
Specificity
PLD2 (Phospho-Tyr169) antibody detects endogenous levels of PLD2 only when phosphorylated at tyrosine 169.
Purity/Purification
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Form/Format
In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Sequence Length
922
Applicable Applications for anti-PLD2 antibody
Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
Application Notes
WB: 1:500~1:1000
IHC: 1:50~1:100
ELISA: 1:10000
IHC: 1:50~1:100
ELISA: 1:10000
Immunogen
The antiserum was produced against synthesized phosphopeptide derived from human PLD2 around the phosphorylation site of tyrosine 169 (E-N-YP-L-N).
All Sites
Human: Tyr169
Mouse: Tyr169
Rat: Tyr169
Mouse: Tyr169
Rat: Tyr169
Preparation and Storage
Store at-20 degree C for 1 year.
Testing Data
(P-peptide-+)
Testing Data
(P-peptide-+)
Immunohistochemistry (IHC)
(Immunohistochemistry analysis of paraffin-embedded human brain tissue using PLD2 (Phospho-Tyr169) antibody. Western blot analysis of extracts from Jurkat cells, treated with TNF (20ng/ml, 30mins), using PLD2 (Phospho-Tyr169) antibody.)
Immunohistochemistry (IHC)
(Immunohistochemistry analysis of paraffin-embedded human brain tissue using PLD2 (Phospho-Tyr169) antibody. Western blot analysis of extracts from Jurkat cells, treated with TNF (20ng/ml, 30mins), using PLD2 (Phospho-Tyr169) antibody.)
Product Categories/Family for anti-PLD2 antibody
References
Steed P.M., FASEB J. 12:1309-1317(1998). Lopez I., J. Biol. Chem. 273:12846-12852(1998). Divecha N., EMBO J. 19:5440-5449(2000).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
104,657 Da
NCBI Official Full Name
phospholipase D2 isoform PLD2B
NCBI Official Synonym Full Names
phospholipase D2
NCBI Official Symbol
PLD2
NCBI Official Synonym Symbols
PLD1C
NCBI Protein Information
phospholipase D2
UniProt Protein Name
Phospholipase D2
Protein Family
UniProt Gene Name
PLD2
UniProt Synonym Gene Names
PLD 2; hPLD2
NCBI Description
The protein encoded by this gene catalyzes the hydrolysis of phosphatidylcholine to phosphatidic acid and choline. The activity of the encoded enzyme is enhanced by phosphatidylinositol 4,5-bisphosphate and ADP-ribosylation factor-1. This protein localizes to the peripheral membrane and may be involved in cytoskeletal organization, cell cycle control, transcriptional regulation, and/or regulated secretion. Two transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Jul 2011]
Uniprot Description
May have a role in signal-induced cytoskeletal regulation and/or endocytosis.
Research Articles on PLD2
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Product Notes
The PLD2 pld2 (Catalog #AAA855468) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The PLD2 (Phospho-Tyr169) Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's PLD2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA). WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:10000. Researchers should empirically determine the suitability of the PLD2 pld2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PLD2, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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