PAK1/2/3 Antibody | anti-PAK1/2/3 antibody

PAK1/2/3 Antibody

Cat. Num. AAA8509494

• Gene Names: PAK1; PAKalpha
• Reactivity: Human, Mouse, Rat
• Applications: Immunohistochemistry, ELISA
• Purity: Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Synonyms: PAK1/2/3; PAK1/2/3 Antibody; ADRB2; Alpha-PAK; CDC42/RAC effector kinase PAK-A; EC 2.7.11.1; P65-PAK; P68-PAK; PAK 1; PAK-1; PAKA; Protein kinase MUK2; kinase PAK1; p21-activated kinase 1; anti-PAK1/2/3 antibody

• Reactivity: Human, Mouse, Rat
• Specificity: PAK1/2/3 antibody detects endogenous levels of total PAK1/2/3 protein.
• Purity/Purification: Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Form/Format: In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Sequence Length: 553

• Applicable Applications for anti-PAK1/2/3 antibody: Immunohistochemistry (IHC), ELISA (EIA)
• Application Notes: IHC: 1:50-1:100; ELISA: 1:1000
• Immunogen: The antiserum was produced against synthesized peptide derived from Internal of human PAK1/2/3.
• Preparation and Storage: Store at-20 degree C for 1 year.

Immunohistochemistry (IHC)

(Peptide-+ Immunohistochemistry analysis of paraffin-embedded human brain tissue using PAK1/2/3 antibody.)

References

Brown J.L., Curr. Biol. 6:598-605(1996). Parrini M.C., Mol. Cell 9:73-83(2002). Beausoleil S.A., Nat. Biotechnol. 24:1285-1292(2006).

NCBI and Uniprot Product Information

• NCBI GI #: 190886457
• NCBI GeneID: 5058
• NCBI Accession #: NP_001122092.1
• NCBI GenBank Nucleotide #: NM_001128620.1
• UniProt Accession #: Q13153; O75561; Q13567; Q32M53; Q32M54; Q86W79
• Molecular Weight: 61,632 Da
• NCBI Official Full Name: serine/threonine-protein kinase PAK 1 isoform 1
• NCBI Official Synonym Full Names: p21 (RAC1) activated kinase 1
• NCBI Official Symbol: PAK1
• NCBI Official Synonym Symbols: PAKalpha
• NCBI Protein Information: serine/threonine-protein kinase PAK 1
• UniProt Protein Name: Serine/threonine-protein kinase PAK 1
• UniProt Gene Name: PAK1
• UniProt Synonym Gene Names: PAK-1

NCBI Description

This gene encodes a family member of serine/threonine p21-activating kinases, known as PAK proteins. These proteins are critical effectors that link RhoGTPases to cytoskeleton reorganization and nuclear signaling, and they serve as targets for the small GTP binding proteins Cdc42 and Rac. This specific family member regulates cell motility and morphology. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2010]

Uniprot Description

Protein kinase involved in intracellular signaling pathways downstream of integrins and receptor-type kinases that plays an important role in cytoskeleton dynamics, in cell adhesion, migration, proliferation, apoptosis, mitosis, and in vesicle-mediated transport processes. Can directly phosphorylate BAD and protects cells against apoptosis. Activated by interaction with CDC42 and RAC1. Functions as GTPase effector that links the Rho-related GTPases CDC42 and RAC1 to the JNK MAP kinase pathway. Phosphorylates and activates MAP2K1, and thereby mediates activation of downstream MAP kinases. Involved in the reorganization of the actin cytoskeleton, actin stress fibers and of focal adhesion complexes. Phosphorylates the tubulin chaperone TBCB and thereby plays a role in the regulation of microtubule biogenesis and organization of the tubulin cytoskeleton. Plays a role in the regulation of insulin secretion in response to elevated glucose levels. Part of a ternary complex that contains PAK1, DVL1 and MUSK that is important for MUSK-dependent regulation of AChR clustering during the formation of the neuromuscular junction (NMJ). Activity is inhibited in cells undergoing apoptosis, potentially due to binding of CDC2L1 and CDC2L2. Phosphorylates MYL9/MLC2. Phosphorylates RAF1 at 'Ser-338' and 'Ser-339' resulting in: activation of RAF1, stimulation of RAF1 translocation to mitochondria, phosphorylation of BAD by RAF1, and RAF1 binding to BCL2. Phosphorylates SNAI1 at 'Ser-246' promoting its transcriptional repressor activity by increasing its accumulation in the nucleus. In podocytes, promotes NR3C2 nuclear localization. Required for atypical chemokine receptor ACKR2-induced phosphorylation of LIMK1 and cofilin (CFL1) and for the up-regulation of ACKR2 from endosomal compartment to cell membrane, increasing its efficiency in chemokine uptake and degradation. In synapses, seems to mediate the regulation of F-actin cluster formation performed by SHANK3, maybe through CFL1 phosphorylation and inactivation. Plays a role in RUFY3-mediated facilitating gastric cancer cells migration and invasion (PubMed:25766321).