p27Kip1 Antibody | anti-p27Kip1 antibody

p27Kip1 (Phospho-Thr187) Antibody

Cat. Num. AAA8510233

• Gene Names: CDKN1B; KIP1; MEN4; CDKN4; MEN1B; P27KIP1
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, ELISA
• Purity: Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
• Synonyms: p27Kip1; p27Kip1 (Phospho-Thr187) Antibody; anti-p27Kip1 antibody

• Reactivity: Human, Mouse, Rat
• Specificity: p27Kip1(Phospho-Thr187) Antibody detects endogenous levels of p27Kip1 only when phosphorylated at Threonine 187.
• Purity/Purification: Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
• Form/Format: In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Sequence Length: 198

• Applicable Applications for anti-p27Kip1 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:500-1:1000; ELISA: 1:10000
• Immunogen: The antiserum was produced against synthesized phosphopeptide derived from human p27Kip1 around the phosphorylation site of Threonine 187(E-Q-TP-P-K).
• Preparation and Storage: Store at-20 degree C for 1 year

Western Blot (WB)

(Western blot analysis using p27Kip1(Phospho-Thr187) Antibody. Line1: The extracts from HeLa cells untreated; Line2: The extracts from HeLa cells treated with EGF. Line3: The extracts from HeLa cells treated with IFN-alpha.)

References

Eguchi H, et al. (2003) Cancer Res; 63(15): 4739-46. Le XF, et al. (2003) J Biol Chem. Connor MK, et al. (2003) Mol Biol Cell; 14(1): 201-13. Fujita N, et al. (2002) J Biol Chem; 277(32): 28706-13. Ishida N, et al. (2000) J Biol Chem; 275(33): 25146-54.

NCBI and Uniprot Product Information

• NCBI GI #: 4757962
• NCBI GeneID: 1027
• NCBI Accession #: NP_004055.1
• NCBI GenBank Nucleotide #: NM_004064.4
• UniProt Accession #: P46527; Q16307; Q5U0H2; Q9BUS6
• Molecular Weight: 22,073 Da
• NCBI Official Full Name: cyclin-dependent kinase inhibitor 1B
• NCBI Official Synonym Full Names: cyclin dependent kinase inhibitor 1B
• NCBI Official Symbol: CDKN1B
• NCBI Official Synonym Symbols: KIP1; MEN4; CDKN4; MEN1B; P27KIP1
• NCBI Protein Information: cyclin-dependent kinase inhibitor 1B
• UniProt Protein Name: Cyclin-dependent kinase inhibitor 1B
• UniProt Gene Name: CDKN1B
• UniProt Synonym Gene Names: KIP1

NCBI Description

This gene encodes a cyclin-dependent kinase inhibitor, which shares a limited similarity with CDK inhibitor CDKN1A/p21. The encoded protein binds to and prevents the activation of cyclin E-CDK2 or cyclin D-CDK4 complexes, and thus controls the cell cycle progression at G1. The degradation of this protein, which is triggered by its CDK dependent phosphorylation and subsequent ubiquitination by SCF complexes, is required for the cellular transition from quiescence to the proliferative state. Mutations in this gene are associated with multiple endocrine neoplasia type IV (MEN4). [provided by RefSeq, Apr 2014]

Uniprot Description

Important regulator of cell cycle progression. Inhibits the kinase activity of CDK2 bound to cyclin A, but has little inhibitory activity on CDK2 bound to SPDYA (PubMed:28666995). Involved in G1 arrest. Potent inhibitor of cyclin E- and cyclin A-CDK2 complexes. Forms a complex with cyclin type D-CDK4 complexes and is involved in the assembly, stability, and modulation of CCND1-CDK4 complex activation. Acts either as an inhibitor or an activator of cyclin type D-CDK4 complexes depending on its phosphorylation state and/or stoichometry.