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Testing Data (P-peptide-+)

Nuclear Receptor NR4A1 Antibody | anti-NR4A1 antibody

Nuclear Receptor NR4A1 (Phospho-Ser351) Antibody

Gene Names
NR4A1; HMR; N10; TR3; NP10; GFRP1; NAK-1; NGFIB; NUR77
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, ELISA
Purity
Affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Synonyms
Nuclear Receptor NR4A1; Nuclear Receptor NR4A1 (Phospho-Ser351) Antibody; Early response protein NAK1; GFRP; GFRP1; HMR; N10; N10 nuclear protein; NAK1; NGFI-B; NGFIB; Nuclear hormone receptor NUR/77; Orphan nuclear receptor HMR; TR3 orphan receptor; anti-NR4A1 antibody
Ordering
For Research Use Only!
Reactivity
Human, Mouse, Rat
Specificity
Nuclear Receptor NR4A1 (Phospho-Ser351) antibody detects endogenous levels of Nuclear Receptor NR4A1 only when phosphorylated at serine 351.
Purity/Purification
Affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Form/Format
In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Sequence Length
611
Applicable Applications for anti-NR4A1 antibody
Immunohistochemistry (IHC), ELISA (EIA)
Application Notes
IHC: 1:50-1:100
ELISA: 1:40000
Immunogen
The antiserum was produced against synthesized phosphopeptide derived from human Nuclear Receptor NR4A1 around the phosphorylation site of serine 351 (L-P-SP-K-P).
All Sites
Human: Ser351
Mouse: Ser354
Rat: Ser350
Preparation and Storage
Store at-20 degree C for 1 year.

Testing Data

(P-peptide-+)

Testing Data (P-peptide-+)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using Nuclear Receptor NR4A1 (Phospho-Ser351) antibody. Nuclear Receptor NR4A1 (Phospho-Ser351) antibody reacts with epitope-specific phosphopeptide and corresponding non-phosphopeptide. The absorbance readings at 450 nM are shown in the ELISA figure.)

Immunohistochemistry (IHC) (Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using Nuclear Receptor NR4A1 (Phospho-Ser351) antibody. Nuclear Receptor NR4A1 (Phospho-Ser351) antibody reacts with epitope-specific phosphopeptide and corresponding non-phosphopeptide. The absorbance readings at 450 nM are shown in the ELISA figure.)
References
Nakai A., Mol. Endocrinol. 4:1438-1443(1990). Chang C., J. Steroid Biochem. 34:391-395(1989). Bondy G.P., Cell Growth Differ. 2:203-208(1991).

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
33,754 Da
NCBI Official Full Name
nuclear receptor subfamily 4 group A member 1 isoform 2
NCBI Official Synonym Full Names
nuclear receptor subfamily 4 group A member 1
NCBI Official Symbol
NR4A1
NCBI Official Synonym Symbols
HMR; N10; TR3; NP10; GFRP1; NAK-1; NGFIB; NUR77
NCBI Protein Information
nuclear receptor subfamily 4 group A member 1
UniProt Protein Name
Nuclear receptor subfamily 4 group A member 1
UniProt Gene Name
NR4A1
UniProt Synonym Gene Names
GFRP1; HMR; NAK1; Nur77

NCBI Description

This gene encodes a member of the steroid-thyroid hormone-retinoid receptor superfamily. Expression is induced by phytohemagglutinin in human lymphocytes and by serum stimulation of arrested fibroblasts. The encoded protein acts as a nuclear transcription factor. Translocation of the protein from the nucleus to mitochondria induces apoptosis. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2011]

Uniprot Description

Orphan nuclear receptor. May act concomitantly with NURR1 in regulating the expression of delayed-early genes during liver regeneration. Binds the NGFI-B response element (NBRE) 5'-AAAAGGTCA-3' (). May inhibit NF-kappa-B transactivation of IL2. Participates in energy homeostasis by sequestrating the kinase STK11 in the nucleus, thereby attenuating cytoplasmic AMPK activation.

Research Articles on NR4A1

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Product Notes

The NR4A1 nr4a1 (Catalog #AAA853492) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The Nuclear Receptor NR4A1 (Phospho-Ser351) Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Nuclear Receptor NR4A1 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), ELISA (EIA). IHC: 1:50-1:100 ELISA: 1:40000. Researchers should empirically determine the suitability of the NR4A1 nr4a1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Nuclear Receptor NR4A1, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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