MEF2D Antibody | anti-MEF2D antibody

MEF2D (Phospho-Ser444) Antibody

Cat. Num. AAA858714

• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, ELISA
• Purity: Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
• Synonyms: MEF2D; MEF2D (Phospho-Ser444) Antibody; MEFD; Myocyte-specific enhancer factor 2D; anti-MEF2D antibody

• Reactivity: Human, Mouse, Rat
• Specificity: MEF2D (Phospho-Ser444) antibody detects endogenous levels of MEF2D only when phosphorylated at serine 444.
• Purity/Purification: Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
• Form/Format: In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Sequence Length: 514

• Applicable Applications for anti-MEF2D antibody: Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
• Application Notes: WB: 1:500~1:1000; IHC: 1:50~1:100; ELISA: 1:10000
• Immunogen: The antiserum was produced against synthesized phosphopeptide derived from human MEF2D around the phosphorylation site of serine 444 (P-V-SP-P-S).
• All Sites: Human: Ser444; Mouse: Ser437; Rat: Ser430
• Preparation and Storage: Store at-20 degree C for 1 year.

Testing Data

Western Blot (WB)

(P-peptide-+ Western blot analysis of extracts from HepG2 cells, treated with forskolin (40nM, 30mins), using MEF2D (Phospho-Ser444) antibody. Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue using MEF2D (Phospho-Ser444) antibody.)

Product Categories/Family for anti-MEF2D antibody

Autophagy antibody; Cancer; Cardiovascular; Cell Biology; Epigenetics & Nuclear Signaling; Developmental Biologys; Immunology; Drug Discovery Products; Metabolism; Neuroscience; Signal Transduction; Stem Cells

References

Breitbart R.E., Development 118:1095-1106(1993). Gregory S.G., Nature 441:315-321(2006). Zhao M., Mol. Cell. Biol. 19:21-30(1999).

NCBI and Uniprot Product Information

• NCBI GI #: 410442515
• NCBI GeneID: 4209
• NCBI Accession #: NP_001258558.1
• NCBI GenBank Nucleotide #: NM_001271629.1
• UniProt Accession #: Q14814; Q14815; Q5T9U5; Q5T9U6; D3DVC0
• Molecular Weight: 49,856 Da
• NCBI Official Full Name: myocyte-specific enhancer factor 2D isoform 2
• NCBI Official Synonym Full Names: myocyte enhancer factor 2D
• NCBI Official Symbol: MEF2D
• NCBI Protein Information: myocyte-specific enhancer factor 2D
• UniProt Protein Name: Myocyte-specific enhancer factor 2D
• Protein Family: Myocyte-specific enhancer factor
• UniProt Gene Name: MEF2D

NCBI Description

This gene is a member of the myocyte-specific enhancer factor 2 (MEF2) family of transcription factors. Members of this family are involved in control of muscle and neuronal cell differentiation and development, and are regulated by class II histone deacetylases. Fusions of the encoded protein with Deleted in Azoospermia-Associated Protein 1 (DAZAP1) due to a translocation have been found in an acute lymphoblastic leukemia cell line, suggesting a role in leukemogenesis. The encoded protein may also be involved in Parkinson disease and myotonic dystrophy. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2012]

Uniprot Description

Transcriptional activator which binds specifically to the MEF2 element, 5'-YTA[AT]4TAR-3', found in numerous muscle-specific, growth factor- and stress-induced genes. Mediates cellular functions not only in skeletal and cardiac muscle development, but also in neuronal differentiation and survival. Plays diverse roles in the control of cell growth, survival and apoptosis via p38 MAPK signaling in muscle-specific and/or growth factor-related transcription. Plays a critical role in the regulation of neuronal apoptosis ().